Organ Differentiation And Morphogenesis Of Potentilla Fruiticosa Golddrop

The rapid propagation system of Potentilla fruiticosa Golddrop was established based on "adventitious bud differentiation and callus induction, subculture proliferation and rooting culture" with potentilla fruiticosa shoots as explants and the best rooting medium for adventitious bud differentiation and callus induction, shoots subculture proliferation and rooting selected through L9 (34) orthogonal test and quadratic orthogonal rotation combination test. The Results are as follows:1. Periodic measurements, analysis and phenophase observations of 3-year-old Potentilla fruiticosa Golddrop.2. The use of four kinds of different growth regulators to Potentilla fruiticosa Golddrop cuttings rooting culture shows that NNA and GGR could promote the rooting rate of the cuttings to 91 percent or more; while ABT and IBA had relatively smaller effect on rooting induction. Selected as the Canadian Softwood fruticosa Rooting of cuttings sticks better than the old. Moreover, as cuttings for rooting culture, young shoots are better than the old ones.3. The optimal medium for adventitious bud induction was found to be MS+6-BA2.0mg/L+NAA0.2mg/L of which the differentiation rate is 51%. MS,6-BA and NAA were the main inductive factors with the effects (MS>6-BA>NAA).4. The optimal medium for adventitious bud differentiation was found to be MS+6-BA2.0mg/L+NAA0.2mg/L of which the differentiation rate was 97%. MS,6-BA and NAA were the main inductive factors with the effects (MS>6-BA>NAA).5. The optimal medium for adventitious bud subculture proliferation was found to be MS+NAA0.6+6-BA2.0+KT0.2 of which the multiples of proliferation in 15 days was 6.12.6BA, NAA and KT were the main inductive factors with the effects (6BA>NAA >KT).6. Variance analysis of rooting on the three hormones (KT,NAA and IBA) showed that KT and NAA had very significant effects (P=0.0001; 0.0010) and the two were the main factors affecting rooting, while with the P value equivalent to 0.4245, IBA could be ignored of its effects. So KT and NAA were selected as the induction hormones. Through multiple comparisons of rooting number and rooting rate, MS+NAA0.1mg·L-1+KT1.0mg·L-1 were identified as the optimal rooting mediums, the rooting number was 8.6 and rooting rate reached 95.33%.7. Variance analysis of callus induction on the three factors (Explants type,2,4-D,6-BA) showed that Explants type and 2,4-D were the main factors of induction which had significant effects, while the effect of 6-BA could be ignored. The optimal medium for callus induction was found to be Buds+MS+2,4-D4.0mg/L+6BA0.5mg/L with the effects Explants type>2,4-D>6-BA.8. Variance analysis of callus differentiation induction on the three factors (NAA,6-BA, KT) showed that all of the three were the main factors of induction which had significant effects (NAA>6-BA>KT). The optimal medium for differentiation induction was found to be MS+NAA2.0mg/L+6BA1.0mg/L+KT0.5mg/L and the differentiation rate was 55%.