Study On Light Adaptation And Propagation Technology Of Phylllitis Japonica

Phyllitis japonicain Harbin has the application values as indoor potting and outdoor greening, and because its leaves remain green in winter, it is an important plant resource of winter greening. While it was susceptible to burn leaves in bright light, and poorly grow in over shading, so its light adaptation and propagation technology became the main factors affecting the application. The related study on them was conducted in the 5 light shading percentage levels of full sunlight, T1 (30%), T2 (50%), T3 (70%), T4 (90%) in Harbin outdoor environment.The results showed that:its optimal light shading percentage was 50% to 70%,and 70% was the best; it was seriously burned in 15d full sunlight with the aboveground part dry; it was inhibited in 30% shading; it spindly growth in 90% shading. The adapbility orders of Phyllitis japonica in different light conditions were as follows:70%> 50%> 90%> 30%> full light. Phyllitis japonica could be available from spore propagation and. vegetative propagation. As for spore's propagation, seedlings could be got in soil culture method and sterile culture method to meet the production requirements. The best training condition for soil culture method was the growing matrix of 4 peat soil:1 vermiculite:1 sand, at 25℃, and the best for spore sterile culture was the improvement knop's liquid medium at 25℃, with the 65.13% of germination rate. Spring is the best reason for vegetative propagation with the survival rate as high as 93.33%.(1) Shading had a significant impact on Phyllitis japonica's leaf biomass:as the increase of the shading intensity, the leaf area increased and so were the fresh weight of per unit leaf area and leaf water content.(2)Its leaf photosynthetic physiology had significant response to different shading:as the increase of shading intensity, the net photosynthetic rate (Pn) gradually decreased; transpiration rate (Tr) raised first and decreased later, ordering:90%<30%<50%<70%; water use efficiency (WUE) decreased; intercellular CO2 concentration (Ci) rised in a slow trend; the daily variation of its leaf net photosynthetic rate under different shading was bimodal curve with a photosynthetic "noon break" phenomenon; the daily variation of transpiration rate showed a single peak curve, without "noon break" phenomenon, but the peak value at different times; the order of the daily average photosynthetic rate was 90%<70%<30%<50% shading; light compensation point (LCP) decreased in gradual with the increase of shading intensity; light saturation point (LSP) rised after an initial drop with the following order:70%<90% <30%<50%.(3) Shading had a remarkable effect on correlative physiological indicators to its leaf:as the increase of shading intensity, chlorophyll a, chlorophyll b and chlorophyll a+b content increased, while chlorophyll a/b value gradually decreased; the activity order of SOD was: 90%<50%<70%<30%; soluble sugar content decreased; soluble protein content was in the trend of decreasing; the order of the relative conductivity (RPP) was:70%<30%<90%<50%; malondialdehyde (MDA) content rised later after a initial drop in the following order:70% <50%<90%<30%.(4) Shading had greater effects on its ornamental characteristics open-field:after 3 months shading, the yellow leaf reaches 50% in 30% shading and all light treatments, with poor ornamental effect and low scores and in 70% and 90% treatments, its leaves were green and shine, instead of yellow, and plant type was elegant and round, with better ornamental effect and higher scores; after shading for 6 months, in various treatments, it had varying degrees of viewing improvement, and old leaves and yellow leaves were gradually replaced by new leaves, improving ornamental effect. (5) Phyllitis japonica's spores growing matrix and temperature selection in soil culture:in the conventional breeding of spores, according to the time of germination and growth process from early to late, the matrixes were ordered as follows:Matrix 3 (4 peat soil:1 vermiculite:1 River sand)> matrix 1 (2 peat soil:1 sand)> Matrix 2 (vermiculite); at different temperatures the orders were:25℃>20℃>15℃>30℃.(6) The germination rate of spores in different matrixes:the spore germination rates were different in culture mediums with different concentrations, and the culture mediums in different salt concentration were ordered:1/8MS autumn> summer.(8)The induced results of different test formula in tissue culture:when explants were cultured in the induction process, there was no sign of induction of proliferation, and they were intact in the medium at first, and then gradually withered which illustrated that its young leaves, stems and buds were hard to be induced successfully, and it was difficult to conduct culture and production tissue with the leaves.