Reviewn On Microorganism Resource Releated To Biomass Saccharification And Ethanol Fermentation

Saccharification and applications of fermentation microorganism are the Core technology areas of the fuel ethanol production, and the conversions of the fuel ethanol are directly affected by the quality of microorganism resources. The microorganism resources employed in this thesis included both commercial strains purchased from China Center of Industrial Culture Collection and breeding strains in our laboratory. The basic informations of the gathered 90 dominant strains were firstly collected and summarized,and based on them,further observations of morphological features together with molecular structure identification were carried out. We established three systems for effectively preservation of the microorganism resources:sand preservation tube, freeze-dry preservation tube and ultra-low temperature tube systems. Optimum long-term preservation systems for different strains were investigated by determining the indications before and after the storage. The main results are listed as follows:(1) We have collected 90 strains:including 40 ethanol fermentation strains,12 strains of Amylase enzyme,23 saccharification strains,5strains of Pectinase enzyme and 10strains of Cellulase enzyme.(2) The feature and morphological characteristics of mould,bacteria and yeast colony were observed.(3) By comparing the morphological changes,viability,Activity peak of Glycosylation enzyme strains and alcoholic strength peak before and after preservation,the results indicated that:a) ultra-low temperature preservation systems is inappropriate for the sporogenous mould,because part of strains survival below fifty percent after six month, saccharification enzyme, Pectinase enzyme and CMC enzyme activity decreased rapidly b) the Viability preservation system is suitable for sporogenous mould c) freeze-dry tube preservation system is Optimal for all the preservations of tested moulds bacteriums and saccharomyces.(4) By phylogenetic analysis of 16S rDNA sequence in the nine bacterics and 26S rDNA sequence in the ten yeasts, the result is the 16S rDNA sequence and 26S rDNA sequence can nearly distingursh the species,but part of sequence can't be found in the GenBank database, the analysis results is changed to the strain sequence homology 100% with known gene. The result revealed that the purchase of commercial strains species are similar with the described,In the preservation process, the strains are not be pollution and variatron.