Anti-pseudorabies Virus Screening Of Chinese Herbal Medicine Preparation

We collected a lot of information and literature, against the pseudorabies virus molecular biology, epidemiology, clinical symptoms, pathological changes in other aspects of the system are reviewed. With a variety of tests against pseudorabies virus screening of Chinese herbs. PK-15 cell lines, including training; PRV's TCID50 were determined; use three methods of medication against the pseudorabies virus screening of Chinese herbal medicine, the first for the first dosing plus virus, the second after the first drug is added to increase the virus, the third is the role of the virus and drugs after a period of time is also added. By observing cytopathic (CPE) and the measured OD value of MTT method, with the formula, according to the degree of CPE and MTT OD values measured to judge the results. Results revealed that the pseudorabies virus in PK-15 cells TCID50 for the 10-3.50.1ml, that is 3162-fold dilution of the virus,0.1ml is equal to 1 TCID50; with drug serum PK-15 cells in the safety of the concentration 1.25%; three kinds of drugs the way the results were different, the first virus for the first dosing coupled to Astragalus, Guanzhong, Shegan, toad venom anti-pseudorabies virus best. The protection rate was 94.08%,93.89%,97.33%,95.12%. Honeysuckle, Grifola second, the protection rate was 65.51%,35.50%. Chrysanthemum, kelp, hypericin and so the worst, the protection rate was 6.70%,17.46%,12.31%. After the first plus the second drug is added to the virus, wild chrysanthemum, Shegan anti-pseudorabies virus and is the best protection rate was 94.18%,94.18%. Banlangen, Guanzhong, Curcuma second, the protection rate was 49.49 percent.50.82%,50.78%. Frondosa, kelp, hypericin and so the worst, the protection rate was 10.49% 5.75% 6.57%. The third is the role of the virus and drugs after a period of time is also added. Chrysanthemum, Shegan, honeysuckle against pseudorabies virus and is the best protection rate was 97.46%,97.57%,80.51%. Banlangen, Prunella second, the protection rate was 47.66%.,49.60%. Astragalus, Guanzhong, hypericin and so the worst, the protection rate was 10.77%,5.27%,4.17%. The outcome of three modes of action, wild chrysanthemum and Shegan drugs to inhibit virus protection on the cells is higher. In this experiment, three different application methods, selection of herbal anti-pseudorabies. Provide a reference for the production practice.