Characterization Of FGSC Isolated From Offspring Of FGSC Transplantation

In traditional view, in neonatal females of most mammalian, oocyte pool with a finite number of oocytes is set, and the pool will not self-renew afterbirth. Our lab reported long-term cultured female germ line stem cells (FGSC) isolated from neonatal and adult mouse ovaries. The FGSC, with normal karyotype and high telomerase activity, expressed both germline markers as well as stem cell markers. Moreover, offspring generated after transplantation of the FGSC into ovaries of infertile mice. The establishment and characterization of the FGSC are critical evidence for breaking the old rules in reproductive biology. We isolated and established FGSC derived from prepubertal offspring of FGSC transplantation. In this research work, we did further in vitro charaterization of the FGSC, and identified their in vivo function via transplantation. The charateristics and function of the FGSC derived from offspring are same as that from wild type mice. We compared microRNAs profiles between the FGSC and ovaries by microarray. Some significantly differential expressed microRNAs have been identified as regulators in stem cell self-renewal, oocytes development and maturation, DNA methylation as well as some other important biological processes. Our research give clues for further research in FGSC and their regulatory mechanisms.