The Hybrid Strains Of Pleurotus Cornucopiae Identified By ISSR

Along with the rapid development of edible fungus industry, the identification of new type of edible fungus have become an important issue in edible breeding of fungus. To efficiently decrease the identify work and cost of the fruiting test and to increase the rate of breeding, the breeders devoted themselves to searching the fast prediction method of identify new strains. In recent years, DNA marker is a new research direction at home and abroad in identifying the same fungus with different names and new breeds. DNA fingerprinting is stable, fast and simple and can provide technology surpport to protect the right of new strains, which can solve current dispute about stain with different names or breed during planting P. cornucopiae process.Inter-simple sequence repeat (ISSR) was used to determine the forty hybrid strains and their parents strains (J1-2, J2-1), Genetic similarity coefficient and the dendrogram were obtained by NTSYS-pc(Version 2.10e)software.11 ISSR primers which could amplified clear and polymorphic bands could be used to amplify the genomes of all these 42 test strains, and to gain the clear and stable fingerprint maps with strong polymorphism. The result showed that the total 81 bands were gained, and the similarity coefficients ranged from 0.4286 to 0.8537. When the similarity coefficient is 0.6680, forty-tow strains separated in 7 clades were different from their diverse parents.The comprehensive traits of hybrid strain and parent strain fruiting bodies were verified by the fruiting test of 42 strains. The result showed that with the reliability and efficiency of ISSR markers at early stage, the method of ISSR marker on identify of hybrid strains of P. cornucopiae was feasible. It could serve as the means of appraising the new breeds and new hybrid strains and genetic analysis of P. cornucopiae strains.In this experiment,94 clear and polymophic bands-amplified by ISSR technique applied to the monad strains of 2 kinds of P. cornucopiae. It could be found that the difference among 16 tested monad stains of J1-2 was larger, the range of the similarity coefficient was between 0.5000 to 0.7660, and the average similarity coefficient is 0.6385. The difference among 16 tested monad stains of J2-1 was also larger, the range of the similarity coefficient was between 0.5532 to 0.8191, and the average similarity coefficient is 0.6688. The experiment showed that ISSR marker could distinguish the difference of monad strains to avoid the blindness and repetitiveness and to effectively reduce the workload.