| Research and evaluation of the genetic diversity of oat germplasm resources played an instructive role in inspecting the origin and evolution of oats, investigation and collection of oat germplasm resources, the formulation of conservation measures of its genetic diversity, and the excavation of new genes and the innovative use of germplasm resources. This paper evaluated the diversity of 74 oat germplasm resources comprehensively from three aspects of morphology, salt tolerance and DNA, and then compared the genetic diversity of this three different aspects. The results show that:1. The genetic diversity values of various oat germplasm agronomic traits were all great, of which, the value of main spike grain weight was maximum, and the value of skin bare was minimum; when compared with coefficient of variation characters, tillers per plant was the largest, and plant height was the lowest2. 74 oat germplasm resources were divided into five groups by cluster analysis of morphological traits. The utility of materials in groupâ…¡was low. Oat materials in groupâ… ,â…¢,â…£,â…¤could be used as parent breeding materials to breed variety of large grain and high-yield, lodging resistance and large grain, high-yield and high-height, more kernels and round layers respectively. Principal component analysis of morphological traits illustrated that principal components of oat germplasm shape diversity were significant, and oat germplasm morphological variation were main caused by main spica grain weight, grain weight per thousand, grain weight per plant and grain shape of oat.3. All lethal salt concentration of 74 kinds of oat seeds was 1.4%-3.8% and its semi-lethal salt concentration was 0.7%-2.6%. The differences of salt concentration among different varieties were significant. Under double salt stress, different degrees of changes in indicators of different varieties, lead to variety in salt tolerance of different oat varieties. The comprehensive analysis of germination index, morphological and physiological indexes of oats treated with 1.0% complex salt concentration showed that: 74 oat varieties were divided into five germplasm groups. The five germplasm groups included three major groups: skin oats with low salt tolerance, including the second and third germplasm group; naked oats of high salt tolerance, including the fifth germplasm group; mixed groups of low or medium salt tolerance, including the first and fourth germplasm groups.4. Oat DNA extracted by plant genome kit was of high quality and high purity. It was amplified by primers Mseâ… and Pstâ… primers with three selective nucleotides added to the 3' end, and the polymorphism rate of the amplified bands was high and its quality was pretty good.5. fragments polymorphic amplified genome of 74 oat germplasm by selected 10 primer combinations, were amplified 784 clear and discernible bands, to record them with 760 polymorphic sites, polymorphic active sites ratio was 96.91%,. AFLP markers Genetic diversity analysis showed that the average Nei's index (H) of oat germplasm was 0.382, the average Shannon's information index was 0.573, the AFLP genetic similarity coefficient among 74 oat varieties was 0.2881-0.9106, showing a high genetic diversity in the DNA level. AFLP marker clustering analysis results showed that 74 oat germplasms can be divided into five categories, classification has a certain relevance with geographical origins and skin bared or not.
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