SNPs Detection Of LXRα And Its Relationship With Carcass And Meat Quality Traits In Qinchuan Cattle

Liver X receptorαplay a dominant role in lipid and carbohydrate metabolism.adiponectin stimulates fatty acid oxidation, decreases plasma triglycerides, and improves glucose metabolism by increasing insulin sensitivity. Adiponectin is also involved in the regulation of energy balance and body weight. This study was aimed to detect SNPs of LXRa gene by PCR-SSCP in 485 QinChuan cattle at age of 18~24 months, The associations between SNPs locus and some meat traits was analyzed using the general linear model (GLM) in SPSS program. The aim was to explore association of the LXRa variations with some meat traits of Qinchuan population, with a view to accelerating the process of Qinchuan steers breeding.The results were as follows:1,Polymorphisms of LXRa exon area and it's associated with carcass and meat quality in Qinchuan catttle. We detected SNPs in all exons of the liver X receptor gene (LXRa) in Qinchuan cattle,The results showed that there was a mutation(Y=T→C)at 2377th bp of LXRa gene ( located exon 3) .Genotypic variation has a significant effect on back-fat thickness, carcass length and marbling score (P<0.05). The carcass length of BB genotype and AB genotype was significantly different from that of AA genotype (P<0.05). The marbling score and back-fat thickness of BB genotype were significantly different from that of AA genotype (P<0.05). It indicated that BB genotype was favorable genotype in this SNP locus and was highly associated with seven meat traits, what suggested that LXRa gene could be studied as a candidate gene for molecular assisted selection(MAS).2,Polymorphisms of LXRa intron area and it's associated with carcass and meat quality in Qinchuan catttle.We detected SNPs in all introns of the liver X receptor gene (LXRa) in Qinchuan cattle,The results showed that there was a mutation(Y=T→C)at 1891th bp of LXRa gene ( located intron 2) . Cattles with genotype AA have higher arcass weight and back-fat thickness than cattles with genotype AB, but It indicated that AA genotype was favorable genotype in this SNP locus and was highly associated with seven meat traits, what suggested that LXRa gene could be studied as a candidate gene for molecular assisted selection(MAS).