| 146 18~20 month-old Qinchuan steers under similar feeding condition were selected randomly and partial section hereditary variation of H-FABP,A-FABP and E-FABP genes were analyzed by PCR-SSCP. The associations between SNPs locus and some meat traits was analyzed using the general linear model (GLM) in SPSS program. The aim was to explore association of the H-FABP, A-FABP and E-FABP variations with some meat traits of Qinchuan population, with a view to accelerating the process of Qinchuan steers breeding. The results were as follows:1,Study on polymorphism of H-FABP gene and its association with some meat traits in Qinchuan steers.The polymorphism in all of 4 extron in H-FABP gene were detected .The results showed that there was a mutation(Y=T→C)at 130 th bp of H-FABP gene extron 1 in Qinchuan steers. Different genotypes of SNP locus was grouped, its relationship with meat traits of Qinchuan steers was analyzed using the general linear model. The crural girth, back- fat thickness, marbling of AB genotype were significantly different from that of AA genotype (P<0.01). The significant diference was found between the genotypes of AB and AA for carcass depth and tenderness (P<0.05). There was a difference between the genotypes of AB and BB for back-fat thickness (P<0.01),as well as between the genotypes of AA and BB for crural girth and marbling (P<0.01). SNP locus of H-FABP gene extron 1th in Qinchuan steers associated strongly with some meat traits, it indicated that H-FABP gene may be a candidate gene for the some meat traits of Qinchuan steers.2,Study on polymorphism of A-FABP gene and its association with some meat traits in Qinchuan steers.Detected the polymorphism in intron 1th, extron 2th, extron 3th and extron 4th in H-FABP gene, The results showed that there were two mutations(Y=A→T)at 2736 th bp in intron 1 and (Y=A→G)at 4272 th bp in extron 4 of H-FABP gene in Qinchuan steers. Detected there genetypes in extron 1th, Analysis of Variance showed that the back- fat thickness of AB genotype and BB genetype were respectively significantly different from that of AA genotype (P<0.01 and P<0.05). The significant diference was found between the genotypes of BB and AA for marbling, tenderness and water holding capacity (P<0.05). It indicated that B allele was favorable allele in this SNPs locus and were high associated strongly with some meat traits, such as, back- fat thickness, marbling, tenderness and water holding capacity. The genotype AA, AB and BB genotype were detected in extron 4th. Statistical analysis showed that AA-type individual backfat thickness and marbling were significantly(P<0.05) higher than the BB-type individual; AA-type individual tenderness was significantly higher than that of individual BB (P<0.01), indicating the SNPs - point of the A allele is improving backfat thickness, marbling and tenderness of the genetic effect. From the above analysis, in Qinchuan steers A-FABP gene can be detected in the presence of SNP locus, and with the backfat thickness, marbling, tenderness and hydraulic system, such as meat traits were significantly associated. The A-FABP gene may be the major gene associated with some meat traits or closely linked with major gene, so it can be used as Qinchuan steers some meat traits of the candidate gene in Qinchuan steers.3,Study on polymorphism of E-FABP gene and its association with some meat traits in Qinchuan steers.The E-FABP gene extron 4th in the Qinchuan steers of all the genetic variation were detected. There was a mutation (Y = C→T) at 2295 th bp in extron 4th of the E-PABP gene. AA and AB two genotypes were detected but BB genotypes were not detected through SSCP analysis. A and B alleles of the frequencies were 0.94 and 0.06, polymorphic information content of 0.11, a low of polymorphism. Analysis of variance showed that there is no significant difference between the genotype AA and AB meat traits.
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