Genetic Variation Analysis On Seedling Aracteristics Among Provenances And Families Of Pinus Tabulaeformis

Pinus tableaformis Carr. is distributed in the north of China'14 provinces (regions), it is one of the timber tree species and the major species for afforestation ,because of rapid growth,good quality and poor resistance .There is 45% of the total area of natural pine forest in Shaanxi While Shaanxi has the most abundant pine genetic resources. With the economic development and nation-building needs, the demand for pine wood is increasing. However, the situation of loss of genetic resources,lack of tree species and por quality were affected planting effect seriously. we should carry out studies on selection of superior provenance,good family and superior individual , improving the genetic quality of pine. That we have to do lots of works on selecting high quality genotype,increasing the frequency of target gene,changing in forest structure,enhancing the genetic capacity of pine group, to select pine'fine lines with good growth,good quality and strong resistance.The open pollinated seeds of 95 P. tabulaeformis families collected form Luo Nan,Huang Long,Tai Bai,Ning Shan,Huang Ling and Fu Xian in Shaanxi province; Seedling cultivation was done in container nursery with complete random block design. Determining Height,Ground-diameter,Needle length,Needle width,Shoot length,Main root length,Shoot fresh weight,Shoot dry weight,Root fresh weight,Root dry weight,The number of side root,Root-shoot ratio,Shoot dry weight/shoot fresh weight,Root dry weight/root fresh weight,Shoot dry weight/shoot length,Root dry weight/root length,Soluble protein content,MDA,SOD on 2-years old seedlings and were studied through variance analysis, genetic correlation analysis,genetic variation analysis. And the superior families and individual were selected through principal component analysis and the method of comprehensive selection index. The main results and conclusions were as follows:1. There were highly significant differences among different families and provenance except ground-diameter and main root length .There was a highly significant difference on the number of side root among families.2. Multi-comparison of 19 characters among provenances demonstrated that the mean value of height,needle length,needle width,shoot length,shoot fresh weight,shoot dry weight,root fresh weight,root dry weight were the greatest in Luo Nan provenances. The mean value of root-shoot ratio and the number of side root in Huang Ling provenance were the greatest, while the same as the mean value of the main root length in Huang Long provenance. There were highly significant differences on soluble protein content among Luo Nan,Tai Bai and Fu Xian provenances. A significant differences were on SOD among Huang Long,Ning Shan,Tai Bai and Huang Ling provenances.A significant differences were on MDA between Ning Shan and Tai Bai provenances.3. The coefficients of genetic correlate of seeding of Pinus tabulaeformis were highly significant on height,ground-diameter,needle length,needle width and the number of side root among families . Phenotypic correlation and genetic correlation are all highly significant positive correlation between height and needle length,needle. The coefficients of genetic correlate between height and needle length,needle were greater than the coefficients of phenotypic correlate of that .The coefficients of genetic correlate about seeding of Pinus tabulaeformis were more than 87.67% among shoot fresh weight, root fresh weight, shoot dry weight and root dry weight. The coefficients of phenotypic correlation were more than 73.8% among root length,shoot fresh weight , root fresh weight, shoot dry weight and root dry weight. Phenotypic correlation and genetic correlation are all highly significant positive correlation among 5 characters above.Phenotypic correlation were highly negative correlation among root-shoot ratio,Shoot dry weight/shoot length,Root dry weight/root length. Genetic correlation were highly positive correlation between root dry weight/root fresh weight and shoot dry weight/shoot fresh weight , between Shoot dry weight/shoot length and Root dry weight/root length.. There is no significant genetic correlation between SOD and other characters.4. Quantitative genetic analysis demonstrated that family heritability and single tree heritability of soluble protein content were greater, which were 0.992 and 0.997 respectively. While family heritability and single tree heritability of main root length were lower, which were 0.105 and 0.136 respectively.And other characters were controlled by medium heritability. Genetic variation coefficient of ground-diameter,needle length,needle width,main root length,the number of side root,Shoot dry weight/shoot fresh weight,root dry weight/root fresh weight were less than 15.604%.The characters of seedling Pinus tabulaeformis were greater, except main root length and needle width, which was showed that selecting superior families was a good method to improve the genetic quality of Pinus tabulaeformis.5. Superior families of seedling Pinus tabuaeformis were evaluated thought principal component analysis. Superior families selected were L33,L49,L44,H34,C1,XY4,H23,T29,Q3,XY(XY4) .According to growth characters and biomass character in index selection. we can determine the index value of and selected 19 superior families, which were L47,L20,L29,L26,L2,L8,L14,L49,L50,L25,L33,L44,L15,T23,C1,T11,XY(HY4),H34 and T19.According to resistance characters in index selection, we can determine the index value of and selected 19 superior families, which were T23,XY(HY4),L26,XY(HY3),H40,L29,XY(6),H43,L49,L44,H46,L20,XY(HY1),H38,L33,H34,T29,H15 and T31。...