The Bacterial Diversity Of Microbial Community Capable Of Lignocellulose Degradation And It's Application During Composting Process

In order to detecting the microbial diversity of the microbial community with high capacity of lignocelluloses degradation, identifying the effect of microbial community used as inoculant on cattle manure composting, the 16S rDNA clone technique combined with plate isolation was used. Diammonium phosphate, urea, potassium sulfate was added in materials at the beginning of composting for investigating the probability of organification of added chemical nutrient and increasing of the total nutrient during cattle manure composting process with the method of comparative trial. Results were as follows.One hundred eighty four clones and 24 typical strains were detected by method of restriction fragment length polymorphism. The sixty four percentage of the closest relative among them belonged to Clostridium, Brevibacillus, Ureibacillus, and Rhizobium through DNA sequence aligment. Further more, the rest of thirty six percentage clones belonged to uncultured bacterium clone.The duration that temperature of compost pile reached 50℃was shorten after the first four days inoculated, and the themophilic phase of temperature of compost pile above 50℃was lengthened by seven days under the condition of inoculated treatment compared with control. The degradation rate of cellulose, hemi-cellulose and lignocelluloses was also increased by 12.8%, 3.51% and 4.78% compared to the control, respectively. The time of nitrogen loss was put off and the lost quantity of nitrogen was decreased by 3.9% under inoculated condition.The content of total nitrogen, total phosphorus and total potassium was highly increased by adding inorganic nutrient compared to the control treatment that inorganic nutrient was not added, and the increase rate were 47.5%, 115.9% and 59.2% respectively. At the end of the fermentation there were 40.0% of the nitrogen, 20.3% of phosphorus and 19.5% of potassium which had been add into composting were converted to organic nutrient.The results showed that there were a lot of microbial species consisted in microbial community with high capacity of lignocelluloses degradation. The quality of compost was improved and the nutrient loss was decreased when microbial community was used as inoculum. The organic nutrient and total nutrient content of compost was increased by adding inorganic nutrient.