The Mutagenic Effects Research Of NitrogenIon Beam Irradiation On Sweet Sorghum

In this study ,the sweet sorghum seeds(keller and della seeds)are provided by the department of plant soil in University of Kentucky . Using different doses of N+ ion beam radiates the dry seeds of the plant materials, analizes the field survival of M1 generation , and the relationship between the main agronomic performance and peroxidase after the N+ ion dose radiation ; Seroious physiological damage causes in-genetic changes,and undesirable growth result in many plants'death, therefore, M1 generation is not selected in this study ,instead the field one ear harvest storage. The following year, using the "ear-line method" planting, M2 on behalf of the traits under field screening mutant phenotype, then the fall harvest one ear test species, agronomic traits under field and laboratory test data types of data analysis, each species selected 12 ( include control lines) excellent mutant for ISSR polymorphism. The results showed that:(1) by different doses of nitrogen ion beam irradiation of sweet sorghum Della and Keller Field survival showed a "saddle type" curve, with the increase of radiation dose, field survival rate increased again after the first drop down.(2) different sweet sorghum have different sensitivities to nitrogen ion beam irradiation. this study shows that the sensitivity of sweet sorghum keller is higher than the sweet sorghum Della.(3) the same species, different irradiation treatment on the M1 generation brings different effect on stem diameter, plant height, node number, yield, sugar hammer degree of performance and peroxidase .(4) nitrogen ion beam irradiation can cause mutations in various strains of sweet sorghum, this study examined a number of stem diameter, plant height, sugar hammer high, early maturity and good dwarf-type mutants. In particular, The growing time of mutation screening of precious dwarf sweet sorghum KM32-3 is shortened by 25 d.(5) the use of phenotypic traits combined field ISSR molecular assisted breeding technology helps improve the efficiency of mutant selection.