MRNA Expression Profile Of FSHβ Gen In Reproductive Cycle In Geese

FSH is a pituitary glycoprotein gonadotropin that is composed of two subunits, the commonα-subunit and the specificβ-subunit, which determines the binding to specific receptors and induction of biological response,and it plays a key role in hypothalamic-pituitary-gonad axis. Wanxi White Geese in this study as the research object, the application of real-time fluorescence quantitative PCR technology to detect gene FSHβmRNA Wanxi White Geese in the pituitary, hypothalamus, ovarian tissue in the pre-three week period on the nest, laying 10 off-days and the expression level of the middle period. Wanxi White Geese of three organizations in the FSHβgene expression of the four period, further study will FSHβgene function, FSHβthe secretion and expression of specific organizations to provide theoretical basis.FSHβgene found hypothalamus, pituitary and ovarian expression of both are in the low expression levels of organization as follows: Pituitary> ovary> hypothalamus, FSHβmRNA among the organizations of the existence of significant differences in the expression of pituitary tissues and ovarian volume are three times the hypothalamus and 62-fold differences were significantly (P <0.01), ovary FSHβmRNA expression in the hypothalamus was higher than but not significantly. Analysis of the different periods of time showed that the expression of FSHβmRNA from high to high to low are: 10 days after laying > a week before laying> nest period> maternity leave. 10 days after laying one of the FSHβgene expression are pre-week break on the nest perinatal period and three times, 37 times and 120 times the differences were significant (P <0.01); Egg production is higher than the previous week in both the expression of the other two periods, significant differences (P <0.05). The same organization at different stages of FSHβmRNA expression analysis showed that the pituitary expression of the four low during the period were: 10 days after laying> a week before laying > nest period > maternity leave, 10 days after laying FSHβthe differences in gene expression significantly higher than the other three periods (P <0.01); Hypothalamus during the four hours followed by low expression on the nest period> 10 days after laying> maternity leave > a week before laying period on the nest FSHβgene expression differences in the amount significantly higher than the other three period (P <0.01); Ovarian expression of the four low during the period were: a week before laying > nest period > maternity leave> 10 days after laying,a week before laying FSHβgene expression differences in the amount significantly higher than the other three period (P <0.01). Ovarian three periods on the nest FSHβgene expression levels were low: the end of the nest, the nest early, mid-term on the nest, nest on the end of the FSHβgene expression was significantly higher than the other two periods (P <0.01 ).Wanxi White Geese laying the week before in FSHβmRNA expression at a fairly high level until the egg up to a maximum 10 days to leave the middle period and declined rapidly on the nest pre-entered on the end of the nest, FSHβmRNA levels increased in the higher level of . FSHβmRNA levels of FSH and the regularity of the changes that the egg close to Wanxi White Geese.