Study On Technique System Of The Tissue Culture In Strelitzia Reginae

The seed segments were used as explants to establish a rapid technique system of tissue culture in Strelitzia reginae, and especially to find out some effective measures to control the browning. Two pathways of regeneration were observed: one was regeneration directly from clustered buds, and the other was adventitious shoots induction from protocorm. The results were as follows.1) The seed segments were used as explants because of their slighter browning.2)The initiation of embryo buds: H + BA1.5 mg/L + ZT1.0 mg/L + NAA0.8 mg/L was the best medium. Had been cultured 30 days, the buds or shoots could be got.3)The initiation of protocorms: the best medium was 2H+BA2.0mg/L + ZT0.5mg/L + NAA0.6mg/L.4)The multiplication of clustered buds: the best medium was H+BA1.5 mg/L+ ZT 1.0 mg/L + NAA0. 6 mg/L. The explants were cultured alternately on the solid medium and on the liquid medium with filter paper, which was the effective measure to prevent the explants from the browning to certain extent. Had been subcultured for the 6th generation, the multiplication was flourishing, and the average rate reached 5.0 per generation.5)The multiplication of protocorms: the best medium was 2H + BA2.0mg/L + ZT1.0mg/L + NAA0.4mg/L. The browning of explants wasrelatively slighter in this pathway. However, the rate of multiplication was lower, averaging 3.9 per generation. Had been cultured on the same medium for 30~60 days, the protocorms differentiated to small bud clusters.6) The suitable medium for the growth of clustered plantlets was H+ NAA0.1mg/L, which could significantly conduce the induction of roots.7) The best medium for the rooting of plantlets was H+IBA0.5mg/L + NAA0.1mg/L+AC0.5g/L. After 30 days, the very small white root tips emerged from most of the plantlets. Just then, the plantlets were transferred on H medium without any growth regulator, and the roots would grow up quickly.8)For nursling of plantlets, the soil of the rotten leaves in the bamboo groves was the best materials, which could conduce germination of fibrous roots effectively.