| In the recent years,Pinus massoniana Lamb which aren't in the epidemic area of Bursaphelenchus xylophilus died in a large area.Finding any Bursaphelenchus xylophilus but Bursaphelenchus mucronatus in the dote.This confirm that the pathogenicity of Bursaphelenchus mucronatus on pine.Whereas,there was no research about the pathogenesis of Bursaphelenchus mucronatus.The study on Isolating hesion from Bursaphelenchus mucronatus,and testing the role of them at the death of pine The main conclusing are as following:1.There are 26 species bacterium and 4 fungus on the surface of Bursaphelenchus mucronatus.Different Microorganism system in different culture stage of Bursaphelenchus mucronatus:the quantity changes of Microorganism on the surface of Bursaphelenchus mucronatus are 30 species at unpurified culture;21 species at first purified culture;20 species at first subculture;18 species at 2-nd;9 species at 3-rd;7 species at 4-th;5 species at 5-ve.There are Bacillus subtilisâ…¡,Bacillus subtilisâ…¥,Stenotrophomonas maltophiliaâ…¢,Pseudomonas fluorescens,Bacillus amyloliquefaciensâ…¡still on the surface of Bursaphelenchus mucronatus.2.screening 9 virulent bacterium by testing pathogenicity of the bacterium metabolite on pine needles of pinus massoniana Lamb.There are Bacillus subtilisâ…¢,Stenotrophomonas maltophiliaâ… ,Stenotrophomonas maltophiliaâ…¢,Pectobacterium cypripedii,Bacillus amyloliquefaciensâ… ,Bacillus subtilisâ…¤,Klebsiella pneumoniaeâ… , Pseudomonas putida,Agrobacterium tumefaciens.3.The bacterium metabolite was separated into protein and nonprotein to poison pine needles,testing the activity changes of PAL,PPO,POD and the total phenols content at the 12h,24h,36h.the consequence indicate the latent infection of pinus massoniana Lamb.the most toxicity treatment is nonprotein of Pectobacterium cypripedii.4.The nonprotein of Pectobacterium cypripedii inoculate on pinus massoniana Lamb. According the activity changes of PAL,PPO,POD and the total phenols content in pinus massoniana Lamb,find higher density,higher toxicity.According Contrast the effect of germ- free- Bursaphelenchus mucronatus and Bursaphelenchus mucronatus,the Bursaphelenchus mucronatus is super- toxicity.This indicate that the Microorganism on the surface of Bursaphelenchus mucronatus paly a necessary role of pathogenesis of Bursaphelenehus mucronatus of causing death of pine.5.The nonprotein of Pectobacterium cypripedii carried by Bursaphelenchus mucronatus cause pine dried-up.Pectobacterium cypripedii is epiphytic on Bursaphelenchus mucronatus,that is a necessary factor of the pathogenicity of Bursaphelenchus mucronatus on pine and a necessary condition of Pectobacterium cypripedii's survivorship... |
