Isolation And Identification Of Duck Hepatitis Virus And Development Of ELISA For Detecting DHV Antibody

Duck hepatitis virus (DHV) is the causative agent of duck viral hepatitis,an acute and fatal disease of young ducklings.Virus isolates was obtained through duck embryo allantoic sac inoculation from duckling liver samples fromHenan provinces in china. All these ducklings were diagnosed clinically dying from duck virus hepatitis.and then, development of ELISA for detecting DHV antibody.and gain some results as follow.Virus isolates was obtained through embryo allantoic sac inoculation from duckling liver samples from HuBei provinces in china. All these ducklings were diagnosed clinically dying from duck virus hepatitis.Through neutralization test,animal recurrence infectation,young duckling protection experiment and observe virus particle by electron microscope. A strain DHV-Ⅰw as obtainted and identified.Using the purified virus as coating antigen, an indirect ELISA method was developed.Optimized conditions were as following.The concentration of virus for plate coating was 7.5μg per well.P/N ration reached maximum under the condition of serum dilution at 1:320,and the demarcation of negative and positive was most obvious at the same dilution.the threshold value of ELISA was 0.2 with OD450.It was found use this method to monitor the antibody's level in the young ducks,this assay is sensisive and specific as well.