| The ET of buffalograss, the drought resistance and the fluorescence intensity of intracellular Ca2+ in different water condition were studied, in order to understand the drought resistance mechanism, screening the resisting drought and saving water cultivars, and breeding new varieties.The ET of 12 buffalograss materials in different water condition and their drought resistance were studied. The results showed that under sufficient irrigation, they had not significant difference among materials (P>0.05) except T09, and before 18 days of the water stress condition, they had significant difference among materials (P<0.05 or 0.01). As the stress of drought was intensified, the ET, soil water content, relative water content of leaves decreased; In contrary, content of PRO, MDA and relative electrical conductivity rate were increased. The Pearson correlation analysis among the ET and physiological showed that they were significantly correlated with each other (P<0.05). With cluster analysis, 12 buffalograss materials were divided into three grades of the drought resistance, the strong drought resistant, the medium drought resistant, and the weak drought resistant.By using nutrient solution culture and simulated water stress by PEG(6000), the drought resistance indexes of roots and leaves of buffalograss under different PEG concentrations were studied. The result showed that the sensitivity of roots are higher than leaves. As the concentrations was intensified, the content of PRO, MDA and ABA were increased, however, the content of SPr, SOD, POD, CAT and GA were decreased. In addition, fluorescent probe fluo-3/AM was loaded into the cell of buffalograss leaves to measure the fluorescence intensity of intracellular Ca2+ using laser scanning confocal microtechnique. When the stress strength is stronger, the fluorescence intensity, namely the content of intracellular Ca2+ is higher.
|
PDF Full Text Request