Comparison Of Cell Immunity Efficiency Between HCLV Bovine Testis Cell Vaccine And HCLV Rabbit Spleen And Lymph Tissue Origin Vaccine

Classical swine fever (CSF) is a highly contagious disease in pigs caused by the CSF virus. It brings a severe economic losses to the worldwide pig farming. In China it is still one of the most severe diseases. Now Both hog cholera lapinized virus (HCLV) rabbit spleen and lymph tissue origin vaccine and HCLV bovine testis cell vaccine were spreadly used to prevent pigs from CSF in pig farming of our country's. But pigs containing high antibody against CSFV still suffer from CSF. In order to estimate cell immunity efficiency of CSFV vaccine, we have done some research on comparing them .In this study, we develop a track observation for some principal references of immunity in order to take a objective compared analysis with HCLV bovine testis cell vaccine group, HCLV rabbit spleen and lymph tissue origin vaccine group A , HCLV rabbit spleen and lymph tissue origin vaccine group B and negative group by the traditional methods of white blood counting, Flow cytometry,lymphocytes proliferation test and ELISA test kit.The results were shown as follows:1 the level of CD3+,CD4+,CD8+ lymphocytes subsets in PBMC On 1,7,14,24 and 35 days after immunization CD3+/lym of HCLV bovine testis cell vaccine group is lower than HCLV rabbit spleen and lymph tissue origin vaccine group A and B ,but there is no significant difference; CD4+/lym from high to low respectively is: HCLV rabbit spleen and lymph tissue origin vaccine group A and B,negative group and HCLV bovine testis cell vaccine group .And there is a significant difference on 1 day after immunization; There is a significant difference on the ratio of CD4+/ CD8+ only for HCLV bovine testis cell vaccine group between different days.And its ratio of CD4+/ CD8+ is lower than HCLV rabbit spleen and lymph tissue origin vaccine group A and B.There is a significant difference on 35 days after immunization.2 Result of lymphocytes proliferation test On 1,3,7,14,24 and 35 days after immunization SI of nonspecific proliferation from high to low respectively is: group A, group B, HCLV bovine testis cell vaccine group and negative group , and there is a significant difference on 3 and 7 days after immunization; After immunization for 7 days SI of specific proliferation from high to low respectively is: group A, group B, HCLV bovine testis cell vaccine group and negative group,and there is a significant difference on 14 days after immunization.3 The level of some cytokine On 1,3,7,14,24 and 35 days after immunization the level of IFN-γfrom high to low respectively is: group A, group B, HCLV bovine testis cell vaccine group and negative group ,and there is a significant difference on 1,3,7,14 and 24 days after immunization;The level of IL-3 from pigs of group A is higher than other group's, meanwhile the level of IL-3 from pigs of negative group is lower than other group's. The level of IL-3 from pigs of HCLV bovine testis cell vaccine group is lower than group B , but after immunization for 7 days it is higher than group B. And there is a significant difference on 1,3,7,14,24 and 35 days after immunization.4 Result of white blood counting there is no significant difference between groups and different days on leucocyte and lymphocyte count .The results demonstrat that compaired with HCLV bovine testis cell vaccine, the pigs vaccinated with HCLV rabbit spleen and lymph tissue origin vaccine contain high CD3+/lym and CD4+/lym, high and stable ratio of CD4+/ CD8+ ,high activity of T lymphocytes and high level of IFN-γand IL-3.So the cell immunity efficiency of HCLV rabbit spleen and lymph tissue origin vaccine is much more obviously .The objective evaluation take a scientific dependence for choosing CSFV vaccine .