| Coprinus comatu was cultivated on a series of compost in which different amounts of the sawdust and wheat bran were replaced by Chamaecrasta nictitans. The substance conversion rules, the yield and qualities effect were analyzed and the nutritive values were also estimated and studied. The main results were summarized as follows:1, in the series of growth substrates in which different amounts of the sawdust were replaced with C. nictitans (A series), Treatment A1 (0% C. nictitans substituting sawdust, CK) had the fastest mycelium growing daily pace, reached to 0.54cm. As yield to say, the average yield of mushroom of Treatment A4(60% C. nictitans substituting sawdust) was 269.2g in every pocket, increased by 7.11%, 39.35%, 45.19%, 52.48% and 73.70% than those of A5(80% C. nictitans substituting sawdust), A3(40% C. nictitans substituting sawdust), A6(100% C. nictitans substituting sawdust), A2(20% C. nictitans substituting sawdust) and A1.As substance conversion to say, the conversion rate of dry matter, Carbon, Nitrogen and Phosphor of A4 were the tallest, distinguishing to 10.77%, 15.11%, 31.29% and 31.21%; Kalium conversion rate of A3 was highest, up to 45.47%. Before covering soil A1 had the highest dry weight reduction of medium and consumption of respiration, reached to 11.47%; they also were highest after the mushroom were picked, distinguishing to 31.75% and 25.55%. The lignin conversion of A1 was 79.21%; the cellulose conversion of A6 was 50.93%, the half cellulose conversion of A3 was 49.74%, higher than the other treatments. Regression analysis revealed significant parabolic relationships between dry matter, Carbon, Nitrogen, Phosphor, Kalium, half cellulose conversion and the degree of C.nictitans substituting for sawdust. Significant linear relationships were observed between dry weight reduction of medium, consumption of respiration, lignin conversion and cellulose conversion.As the nourishment quality of C. comatu to say, the amino acid gross of all treatments had no obvious difference; A3 had the highest grosses of delicious amino acid, sweet amino acid, branched chain amino acid, aromatic amino acid, children amino acid and essential amino acid, distinguishing to 8.45%, 4.55%, 3.52%, 1.64%, 1.56% and 8.79%; its E/N and E/T were also highest; sculpture amino acid content of A4 was 1.57%, higher than the other treatments in series A. The first limiting amino acid of A series was isoleucine. The relative contents of C14:0, C16:0, C16:1 and C18:1 of A3 were highest, which were 0.60%, 13.70%, 0.95% and 5.20%; the relative contents of C18:0 and C18:2 of A5 were highest, distinguishing to 0.90%, 72.15%; the C18:3 relative content of A2 was 1.20%, higher than the other treatments. The relative grosses of saturated fatty acids (SFA) and monounsaturated fatty acid (MUSFA) of A3 were highest, reached to 15.3% and 6.15% respectively; the relative grosses of unsaturated fatty acids (USFA) and polyunsaturated fatty acid (PUSFA) of A5 were highest, up to 76.70% and 70.55%, respectively. The nicotinamide and VB12 content of A2 were highest, reached to 86.9μg/g and 13.6μg/g respectively; the VB2 content of A1 was 1.4μg/g, higher than the other treatments. According to GB 7096-2003, all the fruit bodies of A series can be eaten safely.2, In the series of growth substrates in which different amounts of the wheat bran were replaced with C. nictitans (B series), Treatment B2 (20% C. nictitans substituting wheat bran) had the fastest mycelium growing daily pace, reached to 0.76cm. As yield to say, the average yield of mushroom of Treatment B2 was 232.2g in every pocket, increased by 24.84%, 51.74%, 88.46%, 99.83% and 187.88% than those of B3 (40% C. nictitans substituting wheat bran), B1 (0% C. nictitans substituting wheat bran, CK), B4 (60% C. nictitans substituting wheat bran), B5 (80% C. nictitans substituting wheat bran) and B6 (100% C. nictitans substituting wheat bran).As substance conversion to say, the conversion rate of dry matter and Kalium of B2 were the tallest, distinguishing to 8.86% and 29.19%; Carbon conversion rate of B4 was highest, up to 14.15%; Nitrogen conversion rate of B5 was highest, up to 37.36%; Phosphor conversion rate of B3 was highest, up to 25.20%. Before covering soil B6 had the highest dry weight reduction of medium and consumption of respiration before covering soil reached to 13.50%; they also were highest after the mushroom were picked, distinguishing to 36.03% and 31.72%. The lignin conversion of B1 was 78.25%; the cellulose conversion of B6 was 33.61%, the half cellulose conversion of B2 was 44.10%, higher than the other treatments. Regression analysis revealed significant parabolic relationships between dry matter, Carbon, Nitrogen, Phosphor, Kalium and half cellulose conversion and the degree of C.nictitans substitution for wheat bran. Significant linear relationships were observed between dry weight reduction of medium, consumption of respiration, lignin conversion and cellulose conversion.As the nourishment quality of C. comatu to say, the amino acid gross of B1 was highest, up to 21.98%; its grosses of delicious amino acid, sweet amino acid, sulphur amino acid, branched chain amino acid, aromatic amino acid, children amino acid and essential amino acid were also highest, distinguishing to 7.42%, 4.56%, 1.46%, 3.08%, 1.65%, 1.53% and 8.48%; its E/N and E/T were a little lower than B5, ranking the second. The first limiting amino acid of B series was isoleucine. The relative contents of C14:0 had no obvious difference; the C16:0 relative content of B4 was 12.60%, higher than the other's; the C16:1 relative content of B2 and B3 were highest, reached to 0.50%; the C18:1 and C18:3 relative contents of B2 were highest, 5.15% and 1.80% respectively; the C18:2 of B1 were highest, up to 73.10%. The relative grosses of SFA of B3 and B4 were highest, both 14.05%; the MUSFA relative gross of B2 was 5.65%, higher than the others'; the relative USFA and PUSFA of B1 were highest, up to 79.00% and 74.20% separately. The nicotinamide of B5 was highest, reached to 64.2μg/g; the VB12 content of B2 were highest, reached to 10.5μg/g; the VB2 content of B1 was 1.2μg/g, higher than the other treatments. According to GB 7096-2003, all the fruit bodies of A series can be eaten safely.3,The results of nutrition evaluation research indicated that, of A series, among 6 evaluating indexes, 3 of A6, the essential amino acid index(EAAI), biological value(BV)and nutritional index(NI)of A6 ranked first, distinguishing to 117.2, 116.2 and 68.1; its amino acid score(AAS)and amino acid ratio coefficient score(SRCAA)were 80.4 and 77.6 respectively, ranking second; the chemical score(CS)was 69.8, ranking third. The order's sum A6 was 10, ranking first in the A series, so the comprehensive evaluation was the first. While the CS, EAAI, BV and NI of A1 were 66.8, 73.2, 68.1 and 26.7, ranking sixth; its AAS and SRCAA were 77.6 and 75.0, ranking fourth and third respectively. The order's sum of A1 was 31, ranking sixth in the A series, so the comprehensive evaluation was the last. Of B series, among 6 evaluating indexes, 5 of B5, the CS (63.9), AAS (74.3), EAAI (80.7), BV (76.2) and NI (32.5), ranked first; the SRCAA was 61.8, ranked fourth. The order's sum B5 was 9, ranking first in the B series, so the comprehensive evaluation was the first. While the CS, AAS and EAAI of B4 were 62.7, 72.9 and 78.2, ranking sixth; the BV and NI were 73.5 and 30.6, ranking fourth; the SRCAA was 61.5, ranking fifth. The order's sum B4 was 29, ranking sixth, so the comprehensive evaluation was the last.4, As above, it was feasible to cultivate C. comatu with C. nictitans substituting the sawdust or wheat bran. In the substituting sawdust treatments, the fruit bodies yield of A4 (60% C. nictitans substituting sawdust) was the highest, while the comprehensive nutrition evaluation of A6 (100% C. nictitans substituting sawdust) was the best; As to the substituting wheat bran treatments, B2 (20% C. nictitans substituting wheat bran) had the highest yield, while the comprehensive nutrition evaluation of B5 (80% C. nictitans substituting wheat bran) was the best.
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