Research On The Nucleic Acid Vaccine And Immunity Of Combine CAV,CPV And CDV

The most dangerous infectious diseases for canids was canine distemper, CPV/CAV-2 enteritis and CAV-2 laryngotracheitis . This study desire through multi-gene vaccine to achieve the prevention purpose of CD, CPV, CAV, At the same time, to resolve the problem of the attenuated vaccine return to ancestral and maternal antibody interference. The H gene of canine distemper virus was amplified by PCR and ligated with T vector, and then cloned into pIRESneo expressing vector. Recombinant plasmid pIRCDV-H was extracted and digested with enzymes and sequenced to confirm its rightness. The VP1 gene of canine parvovirus was amplified by PCR and digested by BamH I and Not I, and then cloned into pIRESneo expressing vector. Recombinant plasmid pIRESVP1 was extracted and digested with enzymes and sequenced to confirm its rightness. The fiber gene of canine adenovirus was amplified by PCR and Hindâ…¢. Recombinant plasmid pIRES-F was extracted and digested with enzymes and sequenced to confirm its rightness. Fifteen dogs were injected at 9 weeks of age with increasing doses of pIRCDV-H , pIRESVP1,pIRES-F. All dogs were challenged with virulent CDV, CPV, CAV after the third injection. NAV vaccinated dogs showed a high IgG titer after the second injection and were protected against infection, while the control dogs were fully susceptible. This study demonstrated that vaccines can protect some dogs against virulent.