| Jingyou grape was used for studying the effect of bagging, NAA and ABA treatment for the berry quality. The qualitative and quantitative determination of anthocyanins were analyzed by high performance liquid chromatography-mass Spectrometry (HPLC-MS) in grape skin. We also used real time revers transcription-polymerase chain reaction (RT-PCR) to detect effects of naphthaleneacetic aicd (NAA) and abscisic acid (ABA) on mRNAs transcript level of fourteen structural genes which strictly related to anthocyanin biosythesis.The results showed that bagging retarded growth and mature of grape berries. NAA treatment retarded fruit maturation, and ABA treatment showed the opposite results. NAA treatment suppressed fruit softened, organic acid degradated, and accumulation of reducing sugar and anthocyanins, while ABA treatment enhanced fruit softness, organic acid degradation and accumulation of anthocyanins.There are 16 sorts of anthocyanins in the skins of Jingyou, including 5 sorts of diglucosides, 2 sorts of acetyl-anthocyanins and 5 sorts of coumaryl-anthocyanins. The anthocyanin contents of grape skins increase along with the growth of grape berries, both the categories and contents of each anthocyanins increase. Not only the accumulation of anthocyanins but also diglucosides is enhanced by ABA, while the accumulation of anthoyanins are suppressed by NAA. When grape is overripe, the concentration of anthocyanins decrease, but not all the sorts of anthocyanins decrease.Anthocyanin biosynthetic genes are promoted by exogenous ABA, and NAA treatment shows the opposite result. The expression profiles of most structural genes correspond with the observed changes in anthocyanin accumulation. And they are up-regulated at veraison. CHSs, CHIs, F3Hs, F3'H, F3'5'H, DFR, LDOX, UFGT and OMT dominate the accumulation of anthocyanins and play a role in flavonoid pathway of anthocyanin synthesis. |
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