| Drought resistance of sunflower shoots period, strong or weak, is directly related to the emergence and growth of seedlings, so it is a very important practical significance for the agricultural production of sunflower to identify the drought resistance of sunflower shoots period and research on genetic improvement of sunflower. Recently, the development of molecular markers which speeds up the process of crop breeding provides a powerful weapon. However, the application of molecular markers on the sunflower research began rather late, thus it affects the sunflower breeding for drought resistance process. Based on 11 sunflower breeding materials and K55×K58 hybrid combinations, this paper carries on a preliminary study of the identification methods of sunflower drought-resistance, inheritance of resistance, SSR molecular markers and so on. The main findings are as follows:1. Research on the 11 different drought-resistance of sunflower breeding material through the germination tests indoor and outdoor pot tests to identify 14 indicators related to drought resistance such as determination of the germination index, germination index and plant height. Analysis the relationship of above indicators and drought resistance at seedling stage by use of gray relational grade theory, regard the product of drought index in seed germination and drought seedling survival as Gray Relational Analysis series reference X0, and select eight larger correlative indicators, such as relative germination rate, relative germination index, shoot relative growth rate, root relative growth, relative conductivity, relative water content and so on. Finally, use the membership function to analysis the drought resistance and quantitatively evaluate the drought in the 11 sunflower shoots'period. Select two lager drought separated species K55 and K58 for future experiments.2. The relevant traits'performance of the F2: 3 population and the F1 show that the F2: 3 groups of root and shoot growth traits have the higher coefficient of variation and a larger amplitude, MDA, bud growth and germination rate have the lower coefficient of variation of traits and a smaller variation, and the average performance of the characters of F2: 3 groups were lower than F1 hybrids and their parents. Through the analysis of K55×K58 hybrid combinations of parents, F1, F2 field identification and genetics, the results show that: K58 has drought resistance, K55 has emotional variety, F1 has the high resistance, F2 has resistant and susceptible separation in line with the 3:1 ratio, therefore the resistance of K55 is controlled by1 ~ 2 pairs of major genes, drought resistance is dominant, F2 has dominant performance.3. Establish an anti-drought SSR system used the Single-factor method for sunflower by setting on several levels in the DNA contents and annealing temperature. 2×Taq Master Mix 12.5μl final concentration 1×, DNA template 1μl final concentration <500ng, upstream and downstream primer 0.5μl final concentration of 0.1-1.0μM, the Nuclease-Free Waters complements 25μl.Amplified by using the system, bands are clear and stable, less non-specific.4. Use 60 pairs of SSR primers to amplify genetic parents and anti-sense gene pool of K55×K58 hybrid combinations, the results show that: Between the two parents, seven pairs of primers have clear and stable performance polymorphism, which is scattered throughout the 17 linkage groups, the polymorphism frequency is 13.5%, however, two different pairs of primers exist in anti-influenza gene pool which is related with the sunflower drought-resistance gene.5. Depend on data analysis software MAPMAKER (K55×K58) to statistically analysis F2 single SSR amplification results , it shows that ORS510, ORS 317 are under drought stress MDA chain gene-related sites, and genetic distance is the 6.8cM, 9.7cM.
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