Studies On Extracted Technology Of Bioactive Components From Artemisia L And Effects Of The Components On Nutrient Fermentation

In this study, the orthogonal trail design were adopted to screening of optimum extraction method of Artemisia L extractives, and two Inner Mongolian diary goats with permanent ruminal cannulas were fed a diet with concentrate/roughage ratio of 5:5. Artemisia L extractives were supplemented at 0mg/L, 3 mg/L , 30 mg/L, 300 mg/L , 3000 mg/L into the substrates in vitro batch culture, to study the effects of Artemisia L extractives on ruminal fermentation and biohydrogenation of C18UFA in the rumen of the diary goats. A study on the effects of drying Artemisia L extractives on ruminal fermentation and biohydrogenation of C18UFA in the diary goats was also carried out. The results were shown as follows:1 According to extraction yield the optimal technical condition were that: ethanol 55%, solid:liquid 1:8, extract 2h , extracting temperature 95℃, The dry plaster yields for Artemisia scoparia walolst.et.kit and Artemisia annua L,respectively were 20.4%, and 31.2%.2 when Artemisia scoparia walolst.et.kit extractive were supplemented into the substrates in vitro batch culture, it tended to decrease NH3-N concentration and the ratio of acetate to propionate . Gas production, Acetate, propionate, butyrate and TVFA concentration tended to increase. pH value and degradation rate of substrate DM were significantly increased (P<0.05). hydrogenation of C18 Unsaturated Fatty Acids , proportion of CLA and trans11-C18: 1 tended to increase ,The optimum supplemenation of Artemisia scoparia walolst.et.kit extractive was 3mg/L based a comprehensive analysis.3 when Artemisia annua L extractives were supplemented into the substrates in vitro batch culture, it tended to decrease NH3-N concentration and the ratio of acetate to propionate . pH, Hydrogenation of C18 Unsaturated Fatty Acids, proportion of CLA and trans11-C18: 1 tended to increase. Gas production, degradation rate of substrate DM, Acetate, propionate, butyrate and TVFA concentration were significantly increased (P<0.05). The optimum supplemenation of Artemisia annua L extractive was 3mg/L based a comprehensive analysis.4 when different Artemisia L extractives were supplemented into the substrates in vitro batch culture, and based on pH, Gas production, degradation rate of substrate DM, TVFA concentration and hydrogenation of C18 Unsaturated Fatty Acids. The optimum drying technology for Artemisia scoparia walolst.et.kit was made by concentrated technique, and the optimum drying technology for Artemisia annua L extractive was microwave.To sum up, the optimal technical conditions for extracting bioactive components from Artemisia L were that: ethanol 55%, solid:liquid 1:8, extract 2h, extracting temperature 95℃, and drying by concentrating technique for Artemisia scoparia walolst.et.kit , and by microwave drying for Artemisia annua L extractives. The optimum dose for Artemisia L extractive was 3mg/L.