Intestinal Salmonella Isolation And Identification,as Well As Its Impact On The Endocrine Of Dairy And Study Of Inhibitory Effect

Feces is the main carrier since the microorganism in the livestock is largely discarded through alimentary canal. Salmonella is a kind of Gram-negative bacillus parasitizing in the intestinal tract of the human or animals with the similar bio-chemical reaction and antigen structure.Considering the problems in the treatment of the livestock feces, the isolation and identification of the Salmonella in the dairy cow intestine was studied according to the recent study abroad. And the sterilizing effect of the lime nitrogen to the Salmonella under different temperature and the seasonal change of the Salmonella in the dairy cow intestine were also studied. The study includes,First, 55 Salmonella suspected strains were selected in 200 samples among which 8 Salmonella strains were isolated and the isolation rate was 14.5%. The isolated strains included A group, B group, C1-subgroup and D group among which B group had the largest population, and the following groups were C1-subgroup, D group and A group.Secondly, 38 Salmonella strains were isolated from 350 samples and the isolation rate was 10.9%. The Salmonella detection rate in the June was the highest following which was August, November and December which showed the decline in the Salmonella as the month advanced.Thirdly, Salmonella parasitizing in the dairy cow intestine promoted the release of the ACTH, and inhibited the release of GH and RPL. COR and NE in the blood serum of the cows which had been infected by Salmonella were inhibitedFourthly, fructus mume, baical skullcap and rhubarb had a good bacteriostasis effect on the Salmonella while other chinese herbs had little bacteriostasis on it. Salmonella was hypersensitive to ructus mume and mid-sensitive to baical skullcap and rhubarb.Fifthly, in cattle manure compost, Ordinary temperature was the proper temperature under which Salmonella could not be detected after composting for 3 days with 3% lime nitrogen addition, and 7 days were needed when the Salmonella was killed with 2.5% lime nitrogen addition. While Salmonella in the control group and the group with 2% lime nitrogen addition was still alive.Sixthly, In Co-composting manure and sawdust, Ordinary temperature was the proper temperature under which Salmonella could not be detected after composting for 5 days with 2.5% lime nitrogen addition, and 7 days were needed when the Salmonella was killed with 2% lime nitrogen addition. While Salmonella in the control group and the group with 2% lime nitrogen addition was still alive.