The Effect Of Adding Bacterial Inhibitor To Cattle Manure Composting On Sterilization Impact And Composting Of E.coli

Composting is an effective approach to treat animal manure for its recycling. At present, it was usually using the high temperature to kill the pathogenic bacterium. The temperature was up to 50～60?C, and some of them up to 70?C. But in practice, the temperature was hard to maintain more than 50～60?C in the animal dung compost. This experiment was combined abroad achievement and the practical problem of animal dung in Inner Mongolia. In the condition of low temperature, the experiment mainly investigates on adding bacterial inhibitor ( lime nitrogen) to cattle manure compost on killing effect of colibacillus and compost effect. The result was:1. Pathogenicity E.coli's isolation and identificationThe experiment is investigated on 20 healthy Holstein cow's fresh excreta in different dairy farm in Hohhot. Forty strains E.coli were separated. Six of them were pathogenicity E.coli, and they were virulent strains about 15.0% of all germ. In the six pathogenicity E.coli, one had self-agglutination phenomenon, the other were belonged to O1,O2,O8 serotype. They were about 20.0%,40.0% and 40.0% in typing strain.2. The effect of adding bacterial inhibitor to cattle manure composting on sterilization impact of E.coliThere were five cultivation temperature(20?C,30?C,37?C,50?C,60?C)and two lime nitrogen addition dose(0%,2%), and then determine the optimization compost temperature. Adding different bacterial inhibitor(0%,2%,2.5%,3%), to obtain the optimization addition dose. The result is: 30?C is the ideal temperature to sterilization. In the condition of 30?C, adding bacterial inhibitor to cattle manure. There were no E.coli in 3% and 2.5% field when the time is 48 hours, but there were still E.coli in 2% and 0% filed in the 5 days.3. The effect of adding bacterial inhibitor to cattle manure and serrago composting on sterilization impact of E.coliThere were five cultivation temperature(20?C,30?C,37?C,50?C,60?C)and two lime nitrogen addition dose(0%,2%), and then determine the optimization compost temperature. Adding different bacterial inhibitor(0%,2%,2.5%,3%), to obtain the optimization addition dose. The result is: 30?C is the ideal temperature to sterilization. In the condition of 30?C, adding bacterial inhibitor to cattle manure and serrago. There were no E.coli in 3% field when the time is 48 hours, and there were no E.coli in 2.5% filed when time is 5 days, but there were still E.coli in 0% and 2% filed in the 5 days.4. The effect of adding bacterial inhibitor to cattle manure on compost fermentationThe experiment adopted quiet state compost system, it was draw assistance to ventilation and turning. There were four filed: bacterial inhibitor was 0%,2%,3%,4%, analyzing the variation physical property,temperature,moisture,T-N,NH4+-N,NO3--N and the quantity of E.coli. The result was: lime nitrogen could kill E.coli but could effect compost fermentation. The temperature up to the higher of B,C and D treatment was put off, but the quantity of E.coli was less than the standardization; The temperature of treatment A steped up quickly, but it was still had little E.coli in the end. Increment of T-N in treatment B,C and D was taper, the time to the digest was longer.