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Studies On 12C6+ Irradiated Lilicum Brownii Cv.sorbonne And Lens Culinaris

Posted on:2009-03-06Degree:MasterType:Thesis
Country:ChinaCandidate:Y C ZhangFull Text:PDF
GTID:2143360275495247Subject:Cell biology
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Heavy ion irradiation is very popular used for breeding in recent decades.In this research,dry seeds of lentil,scales and induced shoots of Sorbonne lily were irradiated by penetrating 12C6+ion of different doses.Basic responses of the two species were studied and compared by using various experimental methods.In order to shed light on the breeding of the two species by heavy ion irradiation,the dosage and material selection was also discussed.The major results were as below:A regenerating system was developed by using Sorbonne(Lilium×Sorbonne) scales.The shoot inducing rate was 100%in MS medium supplemented with 1.5mg/L 6-BA and 0.5mg/L NAA.Explants could grow into small bulbs after 50-70days in the medium.Rooting and transition were carried out in pearlite,in which the rooting rate was 100%.Little plants were planted in mixed soil(peat soil:pearlite=1:1).Scales and induced shoots were used in Sorbonne irradiating.Doses used for irradiated scales were 0.7,2.0,4.0,and 6.0Gy,while those used for irradiated shoots were 0.9, 2.7,4.5,6.3,and 9.0Gy.Irradiated scales were found hard to be cultured,the shoot inducing rates were significantly inhibited and the explants grew brown then died not long after the treatment.Irradiated shoots grew well in the medium and showed"up-and-down"trend in their growth as the doses rise,the trend was lost after 5-7 months.Induced shoot irradiating was more suitable for 12C6+irradiating Sorbonne breeding.The survival rate of control Sorbonne shoots was much higher than that of the 12C6+irradiated ones under high concentrations of NaCl stress.NaCl under 1.0% showed no inhibiting effects on the growth of irradiated Sorbonne shoots,but stimulate the growth of 2.7Gy treated shoots.NaCl higher than 2.0%led to great growth inhibition on irradiated Sorbonne shoots,and a large number of the explants died under the stress.We found that the highest variation rate was not induced by the highest dose.Lower doses might be more suitable for inducing inherited mutations in Sorbonne lilies.For the basic components of lentil(Lens culinaris Medic.)seeds were unchanged by the irradiation,no differences in germination rates and plant heights were observed in M1 seedlings.But the treatment led to seeds of the M1 seedlings grew differently by irradiated affection to the embryos of M1 seeds,so that changes were found in germination rates and plant heights of M2 seedlings.Pollen vitality of M1 and M2 seedlings were found reduced as the increased doses.But the gaps between M2 seedlings of each dose were smaller than M1,which implied that self-repair system had repaired some of the damages caused by the irradiation.Yields and protein contents were significantly enhanced by the irradiation in which 90Gy ones had the highest value.M1 seedlings of 90Gy increased 160%of the single plant yield while those of M2 ones had 40%higher yield than the control.The weight increase of M1 seed didn't appear in M2,which implied that the yield enhancement was mainly due to the increase of pods.Some M2 seedlings were found possessing 3-seed-pods,such mutation was very important for lentil breeding.12C6+irradiation caused great changes in protein expression.Some absent and excessive bands were found in all irradiated M1 lentils,those genes coded such proteins might be more sensitive to 12C6+irradiation than others.A great deal of chromosomal aberration and micronuclei were observed in M1 but M2 lentils,the frequencies of each kind of abnormal cells had little changes with the irradiated doses.Sorbonne lilies and lentils were irradiated by different dosages for their different breeding purposes and materials.The responses of Sorbonne and lentil to 12C6+ irradiation were also found in great differences.But the inhibiting effects in Sorbonne growth and the enhancements of lentil yields showed the same"up-and-down"trend.
Keywords/Search Tags:12C6+, irradiation, Lilium×Sorbonne, RAPD, Lens culinaris Medic, growth change, yield, protein content
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