Study On Isolation And Identification Of Lactic Acid Bacteria From Xinjiang Small Reed Silage And Application For Silage Preparation

Xinjiang small reed is widely distributed in Xinjiang and it is one of best forage resources for ruminants.LAB was isolated from manmade Xinjiang small reed silage in this study and we have had cidogenic test for identified strains.Dominant strains were selected and added to the raw materials for silage. At the same time,contrast test with formic acid and commodity silage additive were carried out and the best addition of LAB for Xinjiang small reed silage was determinated.Testâ… : 15 strains LAB were isolated from Xinjiang small silage.According to the results of species formulation and biochemical identification in "Bergey's Manual Determinative Bacteriology" (Eighth Edition),two strains were Lactobacillus pentosus (Hz2-1 Hz2-2),two were Lactobacillus plantarum (Hz3-1 Hz3-2),two were lactobacillus dung (Zar2-8 Zar2-9),two were Lactobacillus brevis(Zar8-3, Zar8-4),three were Lactobacillus delbrueckii subsp .bulgaricus (Gsr1-1 Hz1-1 Zar9-4),two were Pediococcus and two were Lactococcus.Test II: From the results of acidogenic fermentation test with different strains.The Hz2-1,Hz3-1 has a strong ability to produce acid,the pH in Hz3-1 can as low as 3.50 and it was the main strain of produce acid in fermentation of Xinjiang small reed silage.Growth curve of this strain was determinated and found that Hz2-1,Hz3-1 had long growth cycle and achieved stability after 18h.Gsr-t has a short growth cycle while produced acid fast in early period.Testâ…¢: The selected strain Hz2-1(L. pentosus),Hz3-1(L. plantarum),Gsr-t (Pediococcus) were added to Xinjiang small reed for making silage,results show that addition of LAB could improve the fermentation quality of silage,in which Lactobacillus plantarum+Gsr-t had the best fermentation quality.It's pH can up to 3.50.The content of lactic acid was increased by 44% and butyric acid was decreased by 71% compared with the control group,the difference was significant(P<0.01).The content of NH3-N was decreased by 41% and the difference was significant(P<0.01).Formic acid and commodity silage additive had significantly decreased the content of NH3-N,the differences between them was not significant(P> 0.05).Though the results of nutrient composition in Xinjiang small reed silage,we can found that LAB has no significant effect on ADF and EE compare with the control,and the content of ADF and CP were decreased when treated with Lactobacillus plantarum+Gsr-t, the difference was significant (P<0.01), while the content of CP and NDF were decreased and ADF were increased,the difference was nit significant(P> 0.05).Other components had the same effect with Lactobacillus plantarum+Gsr-t.The impact of single strain,formic acid and commody silage additive were not equal to Lactobacillus plantarum+Gsr-t and Lactobacillus pentosus+Gsr-t.Therefore,through the effect of fermentation quality and nutrition content with LAB in Xinjiang small reed silage.We can found that single strain,formic acid and commody silage additive could somewhat improve the fermentation quality of Xinjiang small reed,but the overall effect was not better than Lactobacillus plantarum+Gsr-t and Lactobacillus pentosus+Gsr-t,in which Lactobacillus plantarum+Gsr-t had the best effectiveness and was suitable for fermentation accelerate of Xinjiang small reed silage.