Study On The Relation Between Rhizospheric Soil Enzyme And Microbe In Different Ages Of Tripterygium Wilfordii

Tripterygium wilfordii soil microbe was involved in the formation of soil enzymes and transformation of plant nutrition in Taining County, Fujian Province, which can be used as an important measurement index of the changing in soil organic matter content and soil fertility. In this study, the microbial community structure in 12 Tripterygium wilfordii soil samples at six different locations was analysed using 16S rDNA-PCR-DGGE and ITS rDNA-PCR-DGGE method. Using PCR and denaturing gradient gel electrophoresis (DGGE) assays, we extracted 12 samples of total DNA and analysed bacteria and fungi community composition in these samples. The results showed that six DGGE bands recovered were re-amplified, sequenced and aligned with Blast. five of the targeting fragments belong to uncultivated bacteria, three DGGE bands recovered were re-amplified, sequenced and aligned with Blast. one of the targeting fragments belong to uncultivated fungi, implying DGGE technique having priority in analyzing uncultivated microbe.No significant difference of soil microbial community structure among the Tripterygium wilfordii which was planted in different years in Fujian Province, while differences of microorganism quantities exists, and the overall quantities were increased with Tripterygium wilfordii plant ages. The results also showed that at the same period, the quantity of rhizosphere soil bacteria and fungi is higher than of the Tripterygium wilfordii in FuJian. The urease, catalase, soil-converting enzyme, phosphorylase activity in rhizosphere and non-rhizosphere soil was increased with the overall quantity of fungi and bacteria, showing a positive correlation. Compared with others, non-rhizosphere bacteria of Tripterygium wilfordii planted in 2003 grew better and displayed higher urease activity. The activity of Rhizosphere soil catalase increased rapidly, especially during the period from 2001 to 2003, and the quantity of arhizosphere bacteria and fungi rose quickly simultaneously. Nevertheless, non Rhizosphere soil catalase activity and the amount of non arhizosphere bacteria and fungi went up slowly. Non-rhizosphere soil invertase activity and the amount of non rhizosphere fungi in Tripterygium wilfordii planted during 2003 to 2005 increased in a higher speed than those in Tripterygium wilfordii planted during 2001 to 2002.It is help to investigate the soil microbial activity and soil relations and has important significance to reveal the source of soil enzymes, nature and soil enzymes in the ecosystem role and status of crop cultivation, the cultivation of forests by using advanced research techniques, biochemical techniques and molecular biology techniques. Although more research about the plant and soil microbial enzyme relations is reported, there is no tripterygium research report in this regard.