Analysis On Nutrition Component Of Seed And Extraction, Purification Craft Of Saponin From Gleditsia Iaponica

The resource of Gleditsia Japonica that range from china is rich.The yield of legumen which distributed abroad is large.Saponin is the major valuable components of this kind of plant which was identified as triterpenoid in the previous study.The legumn contains a lot of triterpenoid saponin.The seeds of this plant have sorts of nutritive materials.The aim of the study is to determine the significant nutritional contents of protein components unsaturated fatty acid and linoleic acid. based on the methods of extracting and purifying all kinds of natural crude drug, utilizing:the modern separation technique and the comprehensive evaluation of the legumn of Gleditsia Japonica.The experiment will first extract from,the mountains of acacia for most of saponin and non-saponin part.Saponins part of a purification system,through the silica gel chromatography by the total saponin pure,and the establishment of the mountain acacia saponin content of the standard system.To saponin in the crude rate of indicators,giving full consideration to equipment requirements,environmental protection,the cost of such.factors as the choice of ultrasonic extraction means. Saponins groping in the process of extracting the single factor and analysis.After single-factor test,we draw the basic cost of saponin ultrasonic cxtraction of the best. The marinated time is from 20 min to an hour.Distilling time is 30—60min.the power that we could choose is 50%—80%.The competition is 1/12—1/15.To draw the exact process,the orthogonal experimental design to influence the rate of saponin in the more obvious the extraction time,frequency,solid-liquid ratio for the three factors and three levels of orthogonal experiment.The three factors and three levels orthogonal test to determine the best ultrasound saponin crude extraction process:70 percent ethanol as an extraction agent,apparatus,the maximum power of 80%from 40 minutes ultrasound,the ratio of solid to liquid 1/12.Saponins quality stability experiments show that the total saponin in the mountains of acacia anti-moisture and temperature conditions placed about eight months,the quality can be guaranteed. Experimental work for the above-acacia saponin large-scale production of products has provided technical indicators of reference.The system solvent extraction(ether-chloroform-water-saturated alcohol) and non-polar macroporous resin purification of the crude saponin,DM-130 resin models through screening to determine adsorption,cleared of all in the course of the specific factors data.analysis arid purification of the best draw.Selection of DM-130-macroporous resin,on the kind of concentration of control in the 3-4 mg/ml, pH value raised to 6.0,eluting solvent flow rate of 2-3 ml/min,can be very good absorption effect,Followed by eight-volume of distilled water,two-volume 20 perent ethanol,four-volume 70 percent ethanol to 2-3 ml/min flow rate of elution, collecting 70%ethanol part of eluate,can be purified in Over 70%of the total saponin.Purification of the entire process simple,low-demand equipment purification effect is superior to the traditional alcohol-ether precipitation.Duishan acacia seeds composed of protein analysis to prove their amino acid composition stability,high levels of glutamic acid,to 31%of the total composition. Duishan acacia seed oil to gas spectral analysis to prove that its rich in unsaturated fatty acid and linoleic acid,greater economic value.Saponin in the mountain of acacia. extraction,separation process are two unknown compounds,crystal white,and the structure of identification work is still in progress.