| The reason why the transgenic research of lily is hard to make a breakthrough is that the transgenic acceptor system is too dependent and the gene expression is unstable.The regeneration of lily can be divided into organogensis and somatic embryogenesis.Somatic embryo regenerated system has lots of advantages and it is also a good and attention-worth transgenic acceptor system.Practice proved that it is easy to do transgenic work using somatic embryo.But there is seldom report for somatic embryogenesis of lily,and the rate of somatic embryogenesis of lily is low.This research aims to build a in vitro regeneration system of lily through organogensis and somatic embryogenesis.The result shows as follows:(1)The bulbs of Lilium davidii var.unicolor,'Siberia','White Heaven','Yelloween',and the bulblets of Lilium lancifolium,Lilium sargentiae were selected as explants for diffrentation study,while the regeneration system of L.davidii var.unicolor,'White Heaven','Yelloween' were built.The results of experiments indicated that,for bulbs of L.davidii var.unicolor,the best differentiation medium was MS+6-BA1.0(mg/L,same below)+NAA0.1,while the best mutipulication medium was MS+6-BA1.0+NAA0.5,and the best rooting medium was 1/2MS+ NAA0.8.For bulbs of'White Heaven',the best differentiation medium was MS+6-BA1.5+NAA0.1,while the best mutipulication medium was MS+6-BA1.0+NAA0.45,and the best rooting medium was1/2MS+NAA0.8.For bulbs of'Yelloween',the best differentiation medium was MS+6-BA0.5+NAA0.1+2,4-D0.1,while the best mutipulication medium was MS+6-BA1.0+NAA0.1,and the best rooting medium was1/2MS+NAA0.8.Compared the differentiation rate of them,it showed that low concentration of 2,4-D was benefitial to shoot induction,while high concentration of 2,4-D was benefitial to callus induction.(2)The shoot of L.davidii var.unicolor,'White Heaven' and'Yelloween' were selected as explants for bulblet induction study.The results of experiments indicated that for L.davidii var.unicolor,the best concentration of sucrose was 80(g/L,same below),its inducing rate was 100%,and both of the dimaeter of bulblet and weight of bulblet were the biggest.The inducing rate of'White Heaven' was 100%in the medium with 80 sucrose,while the dimaeter of bulblet and weight of bulblet were the biggest in the medium with 60 sucrose.The inducing rate of'Yelloween' was 100%in the medium with 100 sucrose,while the dimaeter of bulblet and weight of bulblet were the biggest in the medium with 80 sucrose.(3)The result of somatic embryogenesis using the bulb of'White Heaven' indicated that both rate of SE(somatic embryo,same below) induction and average humble of SE induction were highest when the outer bulb of'White Heaven' were used,and induced in dark.The result of orthogonal screening indicated that the best medium for SE induction was MS+6-BA(0～0.5)+2,4-D0.5+LH 400(mg/L,same below),and the best presevation medium was MS+6-BA0.5+2,4-D0.2+LH 1000.the result of browing-preservation experiment indicated that MS medium supplemented with 0.5 6-BA and 4.5g/L AC was best for presevation and proliferatin of SE.(4)The bulbs and leaves of L.davidii var.unicolor,'White Heaven',and the floral organ of L.davidii var.unicolor,Lilium concolor,'Yelloween',and four varieties of Oriental hybrids were selected as explants for EC(Eembryogenic Callus) induction.The results of experiment with bulbs indicated that the best medium for L.davidii var.unicolo to induce EC was MS+6-BA 0.5+2,4-D 0.2,and the best medium for'White Heaven' to induce EC was MS+6-BA0.5+2,4-D 0.5.compared with KT,2,4-D had a better effect in EC induction.The results of experiment with floral organ indicated that using the petal and filament of L. concolor,EC was observed in MS medium supplemented with 0.5 6-BA and 1.0 2,4-D.The result of orthogonal screening indicated that the best medium for EC induction with filament of'Yelloween' was MS+6-BA 0.2+2,4-D0.5+TDZ 1.0,and the best medium for EC induction with pedicel of'Yelloween' was MS+6-BA 0.2+.2,4-D1.0+TDZ 0.1.Because of different genotypes,the rate of EC induction of four varieties of Oriental hybrids was different as well.The results of experiment with leaves indicated that EC of L.davidii var.unicolor was generated in MS medium supplemented with 1.0 NAA and 0.3TDZ,and EC of'White Heaven' was generated in MS medium supplemented with 1.0 NAA and 0.1TDZ.(5)The EC induced by bulbs of'White Heaven' and pedicel of'Yelloween' were selected as explants for SE induction study.The results indicated that the best medium for EC induction with EC induced by bulbs of'White Heaven' was MS+6-BA0.5+LH800,and the best medium for EC induction with EC induced by pedicel of'Yelloween' was MS+6-BA0.5+LH600.
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