Primary Research Into Inhibition Effect And Accelerant Of Endophytic Bacteria And Endophytic Fungi Of Pterocarya SteonopteraC.DC

Endophytes with many varieties are videly distributed, and almost exist in all aquatic and terrestrial plants. The research was based on Pterocarya stenopteraC.DC Isolateded endophytic bacterias from Pterocarya stenopteraC.DC, analyzing antifungal activity of the endophytic bacterias to Fusarium oxysporiumf.sp.melonis. and Erwinia carotovora and investigating stimulative effect to six crop seeds is the aim of this research.. The rusults were as following:1. there are six endophytic bacterias and two endophytic fungi being isolated from stem of Pterocarya stenopteraC.DC .They are Bacillus circulans. Enterococcus gallinarum. Bacillus circulans. Sphingomonas paucimobilis. Burkholderia mallei. Enterococcus faecium. Emericella acristata. Penicillium minioluteum.2. . Fusarium oxysporiumf.sp.melonis. and Erwinia carotovora. were selected as sensitive bacteria to the study,and the rusults showed that amang 8 strains of endophytic bacteria ,there are 5 strains having antifungal activity against Erwinia carotovora and 2 strains having antifungal activity against Fusarium oxysporiumf.sp.melonis. The antifungal activity of FY-3 is the best.3. 8 strains endophytic bacteria were cultivated in distilled water, in centrifugal machine and cell fragmentation machine. The disposaled water was used to cultivate cole, radish and other four kinds of crop seeds.The research showed that through cell fragmentation machine, the seeds's growth were better than other methods. Compared with the other seeds, wheat seed grows the best in distilled water. Radish seed is the best in centrifugal machine and cell fragmentation machine. These six strains seeds get a great growth in cell fragmentation machine. It is presumed that the composed cell supstances have accelerated effect to the seeds.4. It is proved that Pterocarya stenopteraC.DC leaves are provided with antifungal activity.Be tested leaves no matter young or old both have antifungal activity.During these strains, the full-blown leaves's sterilization ability is the best. The Pterocarya stenoptera C.DC leaves have intense sterilization to G+,G-and Microzyme.