Studies On Effects Of NO And Yougui Bolus On Ovulation And Oocyte Maturation And Expression Of NOS In Ovary Of Mouse

Kunming White mice were used as subjects in this study.The effect of L-NAME inhibiting endogenous NO and SNP giving extraneous NO on ovulation and meiotic maturation were analyzed.Immunohistochemistry(eNOS and iNOS) was applied on ovaries of mice,which were given an injection of L-NAME to show how modulation of NO by manipulating NOS substrates affecting mouse follicle growth and ovulation.1.Effects of NO on ovulation and optimal meiotic maturation of immature female Kunrning White mice(18-21 days old) were studied.Mice were given an injection of L-NAME,SNP or co-injection of L-NAME and SNP.RESULTS:50 and 100 mg/kg of L-NAME significantly inhibited the extrusion of first polar body of mouse oocyte(P＜0.01). 1 and 5 mg/kg of SNP had an inhibitory effect on ovulation(P＜0.01).Both of L-NAME and SNP had no inhibitory effect of GVBD.Ovulation using co-injection of L-NAME and SNP was not significant higher than the control,but the extrusion of first polar body of mouse oocyte was significant lower.Suitable concentration of NO play an important part in ovulation and optimal meiotic maturation,to which higher or lower NO would be harmful.2.Immature mice were given an injection of PMSG and hCG.These mice were given an injection of different concentration of L-NAME 3 hours before and after injection of hCG. Immunohistochemistry(eNOS and iNOS) was applied on mouse ovaries to investigate the expression of iNOS and eNOS.RESULTS:Both iNOS and eNOS staining were found within different cell types of mouse ovaries but eNOS immunoreactivity was more intense compared with that of iNOS.iNOS staining was weakly expressed in atresia follicles and hCG induced iNOS expression in ovaries significantly.After 5mg/kg L-NAME treatment,cells of ovaries showed decreased expression of iNOS and negative expression was observed when the treatment of L-NAME was 50mg/kg.eNOS was detected in most cells of ovaries of mice which weren't given an injection of PMSG.After 50mg/kg L-NAME treatment,cells of ovaries showed decreased expression of eNOS and eNOS staining could still be observed within primordial follicles and theca cells after 100mg/kg L-NAME treatment.3.Effects of Yougui Bolus,PMSG and hCG on ovulation and quality of oocyte were studied.Parthenogenetic activation was used to investigate development ability of oocyte. RESULT:Yougui Bolus could play its effect synergistically with western medicine and raise the quality of oocytes.