Study On Seed Priming And The Physiological And Biochemical Change Of Turfgrass

Seven Turfgrass Seeds were used as materials in the experiment,which were Jaguar3, Jaguar4,MiniMustang,Aolikeshi,Heimeiren,Arabiaand Poa pratensis(Football 2) were treat by four priming treatments(Water primimg,PEG priming,Sand priming and Sand+SNP compound priming),and their seed vigor were tested by ways of seedling growth test, accelerated ageing test,cold test,electrical conductivity test,and simulated field seedling growth test,etc.More suitable and effective methods for improve seed vigor of seven Turfgrass were found by analyzed the effect of different suitable methods to the Turfgrass seeds.On the base of it,the change of three enzymes(ADH,POD,Apase) activation with the feasible ways were researched to discuss the effect of priming methods to enzymes activation and physiological and biochemical mechanism.1.The result of regression analysis showed that through seedling growth test,some of vigor indexes were significantly or highly significantly correlated with seedling rate of simulated field condition.For example,germination energy(r was 0.977),germination percentage(r was 0.963) and simple vigor index(R was 0.808).And accelerated ageing test and cold test germination percentage were also highly significantly correlated with seedling rate of simulated(r were 0.949 and 0.933).These showed that seedling growth test, accelerated ageing test and cold test were all better test methods for vigor test of Turfgrass seeds.2.Effect of water priming on seed germination and vigor of Seven Turfgrass Seeds was studied with seedling growth test,electrical conductivity test,accelerated ageing test,cold test and simulated field test.The results showed that vigor of Turfgrass Seeds samples were obviously improved by water priming.Three enzymes(ADH,POD,Apase) activation were higer than comparison.Different Turfgrass Seeds samples had the different time of seed soaking and preserving moisture.Jaguar 3,Jaguar 4 and Mini Mustang were 15min and 24h;Aolikeshi and Heimeiren were 10min and 24h;Arabia was 10min and 12h.;Football 2 was 15rain and 12h.3.The result of PEG priming showed that through priming,germination rate of Turfgrass seeds increased obviously,seedling was doing well,and simple vigor index increased to some extent and so on.In this process of priming,high PEG disposal and short soaking time can give significant effect.There was suitable treatment of PEG concentration and priming time.On the basis of the experiment,the suitable treatment of PEG concentration and priming time of Jaguar 3 and Jaguar 4 were 30%,6h;Mini Mustang was20%,12h;Aolikeshi,Arabia,Heimeiren and Football 2 were 20%,6h.4.The result of sand priming showed that through priming,germination rate of Turfgrass seeds increased obviously,the higher disposal which could improve the Turfgrass's vigor more effect.Mostly results of accelerated ageing test,cold test,simulated field seedling growth test were higer than comparison.Three enzymes(ADH,POD,Apase) activation were higer than comparison.On the basis of the experiment,the suitable treatment of disposals of Jaguar 4 and Mini Mustang were 7.4%;Aolikeshi,Arabia,and Football 2 were 3.8%;Jaguar 3 was 5.7%;Heimeiren was 2.0%.5.Along with the increaseof SNP concentration,the vigor index of seedling growth test, accelerated ageing test,cold test was descendant.Of all the Sand+SNP compound priming disposals,1.0mmol/L and 1.5%mmol/L were the best concentration,which most of vigor index was higer than comparison.Three enzymes(ADH,POD,Apase) activation were higer than comparison.On the basis of the experiment,the suitable treatment of disposals of Jaguar 4, Heimeiren,Aolikeshi,Mini Mustang and Football 2 were 1.0%;Jaguar 3 and Arabia were 1.5%.