Study On The Genetic Diversity Of The Species Of Niupidujuan (Rhododendron Chrysanthum)

Multiplex environmental factors are generally expected to have significant effects on genetic diversity of plant populations. In this study, random amplified polymorphic DNA (RAPD) technique and ISSR technique were used to reveal the genetic diversity in the same species of four indigenous populations collected from different altitudes of Niupidujuan (Rhododendronchrysanthum), a species in danger, endemic to Northeast China. Initially, 120 RAPD and ISSR primers were screened for amplification. Twenty RAPD and then ISSR informative and reproducible primers were chosen for final analysis. A total of 456 clear bands were obtained, including 426 polymorphic ones. Using POPGENE software, the poly rate was calculated to be 94.07% and the evenness of amplified bands for every primer was 6.8, respectively. Additionally, the mean observed number of alleles was 1.7265 with an effective number of 1.3608. An examination of the gene indicated a diversity of 0.2162 with an information diversity index of 0.3313. For these data, the percentage of polymorphic bands was 72.65% clustering blurred analysis was performed with the aid of NTSYS-pc software to define the Nei's gene diversity and the Shannon information diversity index of the four plant populations. The relationships between the genetic diversity indexes and the geographic and climatic factors were estimated using the Pearson correlation with SPSS 11.0 software. The results of the correlation analysis showed that there were significant (P < 0.05) or highly significant (P < 0.01) correlations between each of the genetic diversity indexes and the different temperatures which were mainly caused by the altitude different populations located.