80 arbitrary primers were used to study the characteristics of random amplified polymorphic DNA (RAPD) in eight breeds of domestic Chinese goose (Sichuan White, Taihu, Zi, Huoyan, Yan, Hebei White, Tianfu - and Tianfu 6 ) and a domestic European goose breed (Rhin) was estimated and the rooted dendrogram of the nine breeds of goose was constructed by unweighted pair groop method using aritl~imetic average (UPGMA) in MEGA (molecular evolutionary genetics analysis Verl .01). Twenty-two of the 80 primers tested amplified 186 reproducible fringerment segments in all breeds in which 50 segments are monomorphic, while 136 polymorphic. The result showed, between Chinese and Rhin breed, 122 polymorphic segments were produced, which is 69% of total amplified segments, so the divergence is distinct. And the coefficient of genetic distance between Chinese geese was confirmed by the coefficient of genetic distance, 0.0115-0.1493, the mean of which is 0.0698. Clustering analysis indicated that nine goose breeds can be generally adirided into three large groups: one is Rhin breed alone, one is the two specialized strains, Tianfu - and Tianfu 6 which were breeded after hybridization of five generation between Landaise and Sichuan White. The third groop included alithe other breeds. The result also probide evidences of the applicability of the applicability of RAPD to determining genetic relatedness within and among differemt poultry populations. This technique is efficient to study genetic variation of goose breeds. |