| The acidity alcohol solution agents level in Orthoral test were set for by single-factor experiments The best procedure for extracting the red pigment of Rose by Orthoral test is adding Rose powder with 5 times extraction at 75℃for 2 hours. The pigment being extracted is more pure verified by infrared analysis. The red pigment of Rose is a kind of cyanine compound by spectrogram technology. It has a basal structure like cyaniding, and its subtle chemical structure needs further studied. The effect of pH on nuance is as follow: when pH>5, nuance would move towards purple and be pink. When pH>9, nuance would be dark brown. Metal ion such as Mn2+ and Al3+ might destroy the pigment of Rose, which might make the pigment unstable. However, Pb2+, Mg2+ and Ca2+ had no apparent effect on the pigment. Cu2+, Ba2+ and Fe2+ might protect the pigment from decolor, and stabilize its structure. The suitable conditions to maintain the pigment are low temperature, dark, natural light when we considered the factors such as temperature, light, H2O2, antiscorbic acid , cane sugar, amylum matrix, and metal ion as well as the effect of materials on the retain of pigment. If adding H2O2, the pigment will be destroyed whereas adding antiscorbic acid, cane sugar, amylum matrix, the rate of retain will increase.The red pigment of Rose has inhibited effect on some kinds of strains such as staphylococcus aureus, blastomyces albicans, pseudomonas aeruginosa, klebsiella pneumoniae, especially on staphylococcus aureus, blastomyces albicans. But it has no apparent inhibited effect on bacillus coli and Bacillus subtilis. Distilled water has no inhibited effect on the strains according to negative control experiments. The pigment solution with a concentration of 30 mg/ml has a better inhibited effect on staphylococcus aureus, blastomyces albicans and klebsiella pneumoniae than benzylpenicillin sodium (2000U/ml). The inhibited effect of benzylpenicillin sodium (2000U/ml) on pseudomonas aeruginosa is better than The pigment solution (30 mg/ml). The red pigment of Rose has a strong and stable inhibited effect on staphylococcus aureus. It can be used to develop antibiosis and anti-Inflammation medicine as well as antibiosis antiseptics, toner in food and additives in other products.This experiment showed that the red pigment of Rose had functions of disoxidation and antioxygen according to two antioxygen system vitro experiments. It had a clear effect on superoxide anion free radical produced by autoxidation system of ortho-dihydroxyl and OH produced by FeSO4 and H2O2 reaction. This showed that the red pigment of Rose was a kind of natural edible colorant with perfect antioxygen ability. It could be used to chromatosis food and prevent diseases induced by vivo lipid peroxidation and free radical damaging, delay aging and prevent diseases. Although the extractives from the red pigment of Rose in the two above system have some differences in antioxygen, it certainly reflects a strong antioxygen activity. The possible reason is that different systems have different sensibility.
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