| Millettia dielsiana Harms is a kind of semi-evergreen perennial woody vine plants,belongs to Millettia Wight et Arn.Fabaceae.For the showy flowers and foliages,broadly adaptability,dielsiana is a valuable ornamental plant and germ plasm resource,it would be used popular in gardening as Wisteria sinensis,Campsis grandiflora,Millettia reticulata Benth.In our study,dielsiana were propagated by sexual propagation and asexual propagation,including cutting,layering and tissue culture.Using the LI-6400 portable photosynthesis system,indexes of photosynthesis Characteristics were measured.Application feasibility was also summarized by wild investigation.The conclusions are as follows:1,Photosynthesis Characteristics of dielsiana were primary studied,the result showed that dielsiana is a sun plant,its Light Saturation Point(LCP) is 2000μmol. m-2.s-1and Light Compensation Point(LCP) is 25μmol.m-2.s-1.After shading treatment, LSP decrease rapidly while LCP decrease slowly.The size of leaves increased as the weakness of light strength and thickness decreased,but its growth was well.It can be concluded that dielsiana has stronger adaptation and lower requirement of light.2,Study on adaptability of introduction,transplanting survival rate was higher in early spring at stage of germination and in autumn after growth.Base on the seed introduction,the germination rate of dielsiana in April was 16.67%and in November was 20.51%.3,Cutting rooting performances was observed,the optimal treatment combinations were:30mins of NAA 0.50 mg/L+river sand mixed with garden soil medium, the re-root rate was 60.32%.Compare with cutting,layering rooting was easier and re-root rate was 76.00%.4,Tissue culture was first done on dielsiana.The experiment showed:The best base media was MS+ 6-BA0.50 mg/L+IAA0.10 mg/L,the best media of proliferation was MS+6-BA3.00 mg/L+NAA0.40 mg/L,multiplication rate was 3 to 6 per month.In the process of rooting,rooted-out bottle was better than rooted-in bottle and rooting way two-step,rooting rate was up to 100%.Perlite mixed with river sand and soil was the best transplanting media,transplanting survival rate was 92.83%.5,Biological characteristics,ornamental characteristics and application value of dielsiana were investigated,surveys showed that dielsiana distribute and adapt widely in Hunan province,it has bright-colored flower,beautiful canopy,leaves and fruits,dielsiana is a good ornamental plants with higher ecological and economical value. |
