Studies On The Seed Germinative Features And Tissue Culture Of Herpetospermum Pedunculosum (ser.) C.B.Clarke

Herpetospermum pedunculosum(ser.)C.B.Clarke, a specie of Herpetospermum pedunculosum of cucurbitaceae, is an annual melon climbing plant, with its seed as medicine for the treatment of infectious jaundice hepatitis,cholecystitis and dyspepsia. The methods of its seed germinative features and its tissue culture techniques were systemly studied. It is very important for the protection of the plateau existing natural vegetation and wildlife resources, source of drugs and sustainable use.With its seeds as materials, suitable conditions for seed germination were studied, and germination rate were measured and analyzed. The stem, leaf, petiole and ovary were used as explants to explore the effects of hormone adding on the callus formation and its rapid propagation. The major results are as follow:1.To some degree, the germination of the seed possessed dormancy character, and its optimum temperature was 20℃.2.After soaking in warm water at 50℃and 300mg/L penicillin solution, the seed germination potency and percentage were as much as 12% and 20% higher than the control respectively, and their difference was very significant; 100g/L Polyethylene Glycol solution could also enhanced both of them, 10% and 14% more than the control; 1.0% Hydrogen Peroxide had the positive effect as well.3.It was found by a orthogonal test that the leaf was the optimal explant to induce callus. The induction rate of the leaf was higher than that of the stem obviously, and the callus from the stem was white, Waterlogging shaped, but multiplicated slowly, browned and died gradually; browning and dying occurred in the petiole under the experimental condition.4.The most favorable leaf callus induction medium was MS+2.0 mg/L 6-BA+2.0 mg/L 2,4-D+1.5 mg/L NAA; the ovary was MS+2.0 mg/L 6-BA+1.5 mg/L 2,4-D+0.5 mg/L NAA ; and the callus was MS+1.0 mg/L 6-BA+1.5 mg/L 2,4-D+0.5 mg/L NAA.5. With in-vitro organ culture, it was easier to induce callus from each explant and form adventitious root than to differentiate adventitious buds.