Phenotype Diversity And Gliadin Genetic Diversity Analysis On 23 Brome Perennial Forage In Inner Mongolia

Twenty-three provenances of wild Brome germplasm in mid-eastern Inner Mongolia, were selected investigated in this study. Through phenotype study and seed protein to evaluated genetic diversities of materials, in order to put out the theoretical base for using forage rationally. The main results are as follows:1. Phenotypic diversityPhenotypic diversity was assessed by measuring twelve plant characteristics. The variation coefficient of the ear length(47.40) among the twelve characteristics, is the highest, the lowest is width of glumelle(13.05). According to the PCA, the traits about plant height, spike length, spikelet number, floret number etc, were the main traits for the phenotypic variations analysis. Based on cluster analysis, when GD=21.24 23 provenances were divided into 5 groups by phenotypic traits. The first group was made of five B.ircutensis kom. The second group and the third group were made of all Bromus inermis Leyss. materials. The forth group and the fifth group were all B.ircutensis kom.2. Genetic diversity of gliadinThe genetic diversity of gliadin among 7 B.ircutensis kom. materials and 16 Bromus inermis Leyss. materials were tested by A-PAGE, showed there was height genetic diversity on seed storage proteins, the mean gene differentiation coefficient (Gst) were 0.3749 and 0.4354, indicating that genetic variation within groups were the main sources of total genetic variation.The genetic diversity of gliadin among 23 accessions were tested by A-PAGE, 33 loci were tested totally, in witch 29 loci were polymorphic. The polymorphic rate was 87.88%, witch showed there was height genetic diversity on seed storage proteins. The mean gene differentiation coefficient (Gst) was 0.4841, indicating that genetic variation within groups were the main sources of total genetic variation. According to the UPGAM cluster analysis based on the genetic distance, 23 provenances were divided into five groups(GD=0.18), the different species could poly for one group respective. The first group was made of 17 materials, including two subgroups, the first subgroup was made of B.ircutensis kom. The second subgroup was made of Bromus inerms Leyss. The second, the third, the forth and the fifth group were all made of Bromus inermis Leyss. materials. According the relationship between five genetic diversity parameters and geographic factors, there was no relativity between five genetic diversity parameters and geographic and ecological factors.