Technique For Industrializing Propagation Seeding Of Mulberry

Nong mulberry 14 is a new variety that is high quality,high yield and strongly resisting disease perennial.It was examined and approved by the crop variety examining and approving committee of Zhejiang province.The aim of examination is making the Nong mulberry 14 choiceness new variety rapidly industrialization apply by principium discussing the new variety technique for industrializing propagation seeding.By system study,the mostly conclusions were as follows:1 The result show that mulberry buds of Nong 14 best inducement culture medium is MS+6-BA 3.0mg/L+NAA 0.5mg/L.The succeed medium is MS+6-BA 2.0mg/L+NAA 0.2mg/L.The strong culture medium is MS+6-BA 0.5mg/L+NAA 0.1mg/L.The best rooting culture medium is1/2MS+0.5mg/L IBA.2 The transplanting experiment of tissue culture seeding show that the best transplanting condition is that seeding develop haleness,rooting number is much,root length is at 1.5-2.0cm,temper seeding time is 4 d and transplanting is mud:sand:perlite=1:1:1,transplant temperature is 25℃,humidity is 90%.The transplanting medium is disinfected by the zineb pentagestrone disinfectant.When spray MS+0.1mg/L IBA nourishment liquid in transplanting tissue culture seeding can accelerate seeding growth and strong seeding.3 The experiment of Ex vitro rooting of mulberry tissue culture seeding show that the seeding skewer insert rooting result is best when without root seeding soaked for 30 min in IBA concentration 100 mg/L.The bag of Ex vitro rooting result is best when MS+100 mg/L IBA mother liquid wet transplanting medium.4 Principium discuss that how to reduce culture seeding production cost. Doing tap water replace and doing white sand candy replace cane sugar distilled water and inositel can reduce or taking out from organic thing during doing rooting culture medium,these can greatly reduce culture seeding production cost.5 The key factors that influence on mulberry tissue culture seeding benefit,the basic establishment and instrument equipment and manage were discussed.The production technique flow of mulberry tissue culture industrializing propagation was established.