| Copper is one of the necessary trace elements for plant nutrition, is also one of the important elements necessary for human health. It has important significance to improve the quality of the crop and restore the copper polluted soil that how to improve the growth and the quality of the crop in the copper polluted soil through the study on the movement of copper in soil, its biological effects and the growth of the crop and their accumulation of copper. In this paper, indoor simulation and a pot experiment were used to study systematically on the translation of copper in brown soil and Bordeaux mixture polluted soil and the effect of Brown coal which apply to the copper polluted soil, Alfalfa, a common kind of herbage in orchard and Wu Yue Man Little Cabbage were used to study on the effect of copper on them and their accumulation of it, and the effect on soil enzyme activity. The main results were as follows:1. Soil column was used to study the characteristics of the movement of copper in uprightness direction in brown soil which was in one year rainfall condition in taian. The result showed that the higher the content of copper in soil, the more leaching loss, the content of SUB-Cu and RES-Cu increased by 28.57% to 33.49%. In the soil of high content of copper, the proportion of EX-Cu decreased by 9.97% to 10.48% and the movement of copper decreased by 15.54% with the content of calcium increasing; with the increasing of brown coal input to soil, the content of EX-Cu in the surface layer decreased, the leaching loss of copper decreased distinctly which moved to the layer of 5-10cm and the content of OC-Cu increased by 22.76% to 36.07%.2. With the content of copper increasing, the content of it in little cabbage increased significantly. When the content of copper applied in the soil was less than 100mg·kg-1, the growth of little cabbage increased significantly. In the treatments with high content of copper or with the application of brown coal, little cabbage growed hardly or even died. With the application of calcium, the weight of the little cabbage increased by 81.44% to 380.76%, the content of active copper decreased by 7.56% to 32.51%, the content of total copper decreased by 6.57% to 46.47%, the content of nitrate decreased by 40.20% to 60.51%, the content of VC increased by 8.91% to 27.72%, the content of chlorophyll increased by 29.17% to 58.33%, POD activity increased by 23.53% to 58.82%, CAT activity increased by 16.67% to 200%; NR activity increased by 19.63% and 25.15% at the two lower level of calcium application. With the application of brown coal, the activity of POD increased by 23.53% to 50%. With the application of brown coal, the content of VC increased by 17.82% to 28.71%, the content of active copper decreased by 47.45% to 61.51%. When the contents of brown coal were 10g·kg-1 and 20g·kg-1, the contents of chlorophyll increased by 4.17% and 16.67%, the contents of total copper increased by 14.43% and 6.44%. When the content of brown coal was 5g·kg-1, the activity of CAT increased by 34.62% and the content of nitrate decreased by 8.16%.3. With the content of copper increasing, the weight of alfalfa showed a reducing trend, the content of total copper in it increases significantly. The weight of alfalfa increased by 66.67% to 240% with the application of calcium. In the treatments with the application of brown coal, alfalfa were low and short and their roots growed hardly. With the application of calcium, the relative of chlorophyll increased by 13.16% to 23.68%, the activity of NR increased by 2.78% to 41.58%, the activity of POD decreased by 8.68% to 25.75%, the content of nitrate decreased by 36.56% to 46.44%, the content of total copper decreased by 39.32% to 62.53%; the activity of CAT decreased by 50% when the content of calcium was 0.7813g·kg-1; when the content of calcium was 3.125g·kg-1, the content of crude protein decreased by 41.27%. With the application of brown coal, the content of total copper increased by 8.83% to 20.04%, the activity of POD increased by 14.37% to 17.96%, the content of nitrate decreased by 9.11% to 37.78%; the activity of CAT increased by 104.17% when the content of brown coal was 5g·kg-1; the activity of NR decreased by 10.81% when the content of brown coal was 10g·kg-1; the content of crude protein increased by 30.16% or 12.70% when the content of brown coal was 10g·kg-1 or 20g·kg-1.4. To the little cabbage pot soil, with the content of copper increasing, the activities of the four soil enzymes were all presenting a dropping trend. With the application of calcium, activity of invertase increased by 42.11% to 171.93%, catalase activity increased by 15% to 25%, activity of urease increased by 36.84% to 110.53%, activity of phosphatase increased by 13.95% to 23.26%; with the application of brown coal, activity of invertase increased by 17.54% to 31.58%, activity of catalase increased by 0.83% to 10%, activity of urease increased by 12.12% to 21.21%; activity of phosphate increased by 9.30% and 6.98% when the content of brown coal were 10g·kg-1 and 20g·kg-1. To the Alfalfa pot soil, with the content of copper increasing, activities of invertase and urease both presented a decreasing trend, activities of catalase and phosphatase increased after the first decrease; with the application of calcium, activity of catalase decreased by 17.78% to 32.22%, activity of urease increased 12% to 21%; activity of invertase increased by 4.11% to 6.85%; with the highest content of calcium, activity of urease increased by 16.67%; with the application of brown coal, activity of urease increased 12.12% to 21.10%; activity of invertase increased by 5.48% and 13.70% when the content of brown coal in soil were 5g·kg-1 and 10g·kg-1.
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