Study On The Antioxidance Effect Of Hesperidin And Chlorogenic Acid And On Performance Of Piglets

Two experiments were conducted to investigate the effects of hesperidin and chlorogenic acid on antioxidance function and performance of piglets.EXP.1 was performed to test the scavenging effect of hesperidin and chlorogenic acid on·OH and O2-in vitro.The methods of pyrogallol autoxidation and fenton reaction was used to test the scavenging effect of hesperidin and chlorogenic acid on on·OH and O2-compared with ascorbic acid.Its IC50(concentration of hesperidin,chlorogenic acid and ascorbic acid in the reaction system which can scavenge 50 percent of free radicals) was calculated according to scavenging ratio.The results showed that:hesperidin and chlorogenic acid can scavenging oxygen free radical successful,and a dose-response relationship was observed.(1) The IC50 of hesperidin,chlorogenic acid and ascorbic acid on O2-was 0.6430 mg/ml,0.3691 mg/ml,0.1723 mg/ml.we can concluded that scavenging ability on O2-of ascorbic acid was superior to hesperidin and chlorogenic acid.(2) The IC50 of hesperidin,chlorogenic acid and ascorbic acid on·OH was 5.3704 mg/ml,0.3973 mg/ml,0.4449 mg/ml.we can concluded that Scavenging ability on·OH of chlorogenic acid was superior to ascorbic acid and hesperidin.EXP.2 was conducted to study the effect of hesperidin and chlorogenic acid on growth,antioxidance and immunity of weaned piglets.24 crossbred piglets,(28+ 2)day of age,were randomly allotted into 4 treatments with 3 replicates.The basal diet was corn-soybean meal,which was fed to the 1st group as the negative control. The 2nd group was fed with basal diets adding aureomaycin in 250mg/kg as the positive control.The 3rd and 4th group were fed with basal diets adding hesperidin in 300mg/kg and chlorogenic acid in 300mg/kg respectively.The trial lasted for four weeks.The results showed that:(1) Hesperidin and chlorogenic acid had good effect on growth of weaned piglets Dietary hesperidin had significant effect of the ADG (P＜0.05) and diarrhea rate(P＜0.01) compared with control group during phase 1,but had no significant diversity compared with aureomaycin and chlorogenic acid. Compared with control group during the whole phase,.chlorogenic acid increased piglets average day gain 4.3%,average day feed intake 1.39%,feed conversion ratio 2.75%.(2) Hesperidin and chlorogenic acid had good effect on antioxidance of weaned piglets.Chlorogenic acid increased serum CAT,GSH-Px concentrations and the CIHR(P＜0.05).Hesperidin significantly reduced the contents of MDA(P＜0.05). (3) Hesperidin significantly increased the contents of IgG,IgM(P＜0.05) to improve the Immunity of weaned piglets.In concluding,hesperidin and chlorogenic acid can could obviously scavenging the oxygen free radicals by laboratory experiments and animal experiments.,reliefing oxidation stress of piglets,chlorogenic acid improved the growth by enchancing the antioxidance of piglets.Hesperidin improved the growth by enchancing the antioxidance and Immunity of piglets.