Study On Pathogenesis Of In The Cow Suffered From Alcohol Positive Milk

Alcohol-positive milk (APM) is a nutritional and metabolic diseases that endanger the Chinese dairy cattle industry. But so far, the disease etiology and pathogenesis remains unclear. In order to further study the pathogenesis of APM, this study detected the liver function, renal function, ion metabolism, the microelement level, and the oxidative and antioxidative function and stress-related hormones in dairy cattle with APM during the entire lactation period.This will provide the theoretical support and reference for the prevention and control of APM. The results were as follows:1. In the serum and whey, Ca2+ concentration in dairy cattle with APM was significantly higher (P<0.05;P<0.01)than that in the normal group, and K+ and Na+ concentrations were not significant difference (P>0.05) between the two groups. Mg2+ concentration of the serum in dairy cattle with APM was higher(P>0.05) than that in the normal group, but Mg2+ concentration of the whey in dairy cattle with APM was significantly higher(P<0.05) than that in the normal group, The change in three lactation periods was same. These indicated that the excessive Ca2+ and Mg2+ balanced out the surface charge of casein protein particles, further caused the decreased stability of casein protein, and finally resulted in the form of the APM because of the increased aggregation of the emulsoid.2. GPT and GOT contents in serum in dairy cattle with APM were significantly higher (P<0.05)than that in the normal group. TP and ALB contents in serum in dairy cattle with APM were significantly lower(P<0.05) than that in the normal group. CHOL content was not significant difference (P>0.05) between the the two groups. These indicated that the abnormal liver function could cause the body's metabolic disorder, affect the breast tissue metabolism, and increase the occurance of APM.3. BUN contents in serum in the cow with APM were significantly higher (P<0.05)than that in the normal group. CHOL content was not significant difference (P>0.05) between the the two groups. The change in three lactation periods was same. BUN and CREA is the product of protein catabolism, and react the glomerular filtration function. These indicated that in dairy cattle with APM, the protein catabolism imcreased and the glomerular filtration function decreased.4. INS contents in serum in the cow with APM were significantly lower (P<0.05)than that in the normal group. INS contents in serum were significantly lower (P<0.05)than that in the normal group. The change in three lactation periods was same. These indicated that the utilization of the BLU decreased in dairy cattle with APM. 5. ACTH, CORT and FT3contents in serum in the cow with APM were higher (P<0.05;P<0.01) than that in the normal group. TSH and FT4 content were not significant difference (P>0.05) between the the two groups. The change in three lactation periods was same. These indicated that the cattle in two groups were both in the cold stress situation, but the reaction of the cattle with APM to the environment was more dramatically than that in the normal group.6. In the serum and whey, Zn and Cu contents in the cow with APM were significantly or extreme significantly lower than that in the normal group. TSH and FT4 content were not significant difference (P>0.05) between the the two groups. Fe concentration of the serum in dairy cattle with APM was higher (P>0.05) than that in the normal group but Fe concentration of the whey in dairy cattle with APM was significantly higher(P<0.05) than that in the normal group. The change in three lactation periods was same. These indicated that the metabolic disorder of Zn, Cu and Fe in dairy cattle with APM could cause the decrease of the antioxidase activity, and further cause the body's oxidative damage. The decrease of the Zn and Cu content maybe make the body's immunity and disease resistance decrease, and this make the cattle more easily to be ill.7. In the serum and whey, SOD and GSH-Px activity in the cow with APM were lower (P<0.05;P<0.01) than that in the normal group. ROS and MDA contents were higher(P<0.05;P<0.01) than that in the normal group. T-AOC of the serum in dairy cattle with APM was higher (P>0.05) than that in the normal group , but T-AOC concentration of the whey in dairy cattle with APM was significantly higher(P<0.05) than that in the normal group. The change in three lactation periods was same. These indicated that the body was in the state of oxidative stress, especailly the mammary gland. The accumulation of the excessive free radical caused the oxidative damage to the mammary gland cell and made it secrete the abnormal milk.