The Research On Relationship Between Alcohol Positive Milk And Mammary Gland Epithelial Cells Structure In The Cow

The changes of free radicals metabolism and membrane ingredients and morphology of mammary gland epithetical cells in cow suffered from Alcohol Positive Milk (APM) were studied in this paper for studying the molecular mechanism of APM/s occurring further. The concentrations of free radicals and anti-oxidation indexes were tested. Mammary gland epithetical cells were taken to analyze the phospholipids ingredients. SDS-Polyacrylamide gel electrophoresis was used for analyzing the membrane protein ingredients.ATPase activities which have important function in mammary protein were also tested. Mammary gland tissues were picked up to observe the conventional structure and ultrastructure. Based on the cause of alcohol pisitive milk(APM) and the research we have finished,we make up the compound drug"SUANRUJING"。The experimental cows in the study were devided into three groups,one group as negative group, the second group was fed"suanrujing"as treatment group, one package a day for 5 days while the third group continue to fed 1/4 for another 10 days on the basis of treatment group.The milk quantity and treatment were tested. The results show:1. Although many researches showed that APM is a systemic illness that reacts in part, previous researches only tested some changes in blood indexes. The study showed that the concentrations of ROS and MDA in cows suffered from APM increased significantly while the ability of anti-oxidation in the mammary gland itself is rather lower. Activities of GSH-Px and T-AOC go down appreciably, but not significantly, compared with the cow in control group.2. Five main bands were separated by SDS-Polyacrylamide gel electrophoresis. Compared with the control group, the comparative contents of bandⅠi ncrease significantly, while the comparative contents of bandⅡand bandⅣd ecrease significantly in the cow suffered from APM. The HMP increased, the result from the polymerization of membrane protein. Meanwhile, The comparative contents of the phospholipids ingredients decrease significantly, especially phosphatidylserine (PS) and phosphatidylethanolamine (PE). This may be resulted from the excess free radicals, which can attack the membrane, leading to the changes of protein and phospholipids ingredients in membrane.3. The protein activity with important biology function went dowm remarkly because of the free radicals'attack on membrane protein, especially ATPase activities related to energy and ions metabolism went down significantly. The changes of the phospholipids ingredients also can reduce the ATPase activities that depend on them.4. The changes of membrane ingredients induce the morphological and functional change. It appeared that the epithelial cells of mammary gland suffered blister denaturalization, mitochondria is intumescent, the number of the mitochondrial crests decrease, there were many vacuoles in the endoplasmic reticulum, membrane is absent, the microvilli on the membrane decrease. It was concluded that the produce of APM was due to the membrane ingredients changes and morphological changes, which made the fluidity and changeability of membrane decreased and its permeability disordered that leading to the secretion of APM.5. Compared with the control group, the milk quantity and the anti-oxygen function were improved significantly on the fifth day after the utility of drug, but the effect rebounded on the 15th day in treatment group while the prevention group went on improving. Both groups rebounded on the twentififth day, but the prevention group remained a higher porformence than the APM without treatmen.It was indicated that the drug can prenvent and treat the APM, but it needed to be addied for a long time.