Study On Culture Of In Vitro Rooting Of Almond

In this paper, several in vitro rooting techniques were valuated for micro propagated shoots of almond cultivars, Nong mei"and Chao mei". The results showed that:1. Strengthen the shoots, it reveals that the strong sprout raise can promote the length and thick of stem and provide the strong sprout to taking root for the next step. The best strengthen the shoots technique of Nong Mei is cultured on G+ BA 0.2mg/L+ GA30.5 mg/L; the best strengthen the shoots technique of Chao Mei is cultured on G+ BA 0.5mg/L+ IBA 0.1 mg/L.2. The effect of the kind of growth hormone on rooting of almond, it reveals that these two varieties have the selectivity to the growth hormone type; IAA takes the non-promoter action to these two varieties in their rooting, IBA takes the promoter action to these two varieties in their rooting. The best concernment of IBA for Nong Mei is 1.0mg/L; the best concernment of IBA for Chao Mei is 0.6mg/L.3. The effect of high IBA concernment on rooting of almond shoots, the results showed that the suitable IBA concernment for Nong Mei is 100mg/L and the suitable IBA concernment for Chao Mei is 120mg/L.4. The effect of different temperature on rooting of almond shoots, it reveals that the suitable temperature for these two varieties is 28℃.5. The effect of different media on rooting of almond shoots, it reveals that the suitable media for these two varieties is 1/2MS.6. Dark treatment is the necessary condition; the suitable days of dark treatment for these two varieties is 9d. Dark treatment could be replaced by in vitro basal shoot darking which the rooting percentage was slightly lower, but the rooted shoots were healthier and stronger.7. Low concentration of AC has no influence on rooting of almond, but it obviously enhances the number of root. Highly concentrated AC has suppressed almond's taking root.8. The effect of different concentration of sucrose on rooting of almond shoots, it reveals that the suitable concentration of sucrose for these two varieties is in 20g/L-25g/L.9. Planting, the most crucial step of planting is to transplant the little plant live from sterile medium to matrix, to water nourishing oral liquid, to keep matrix moisture.