Study On Cloning And Expression Of Pig MEF2A Gene

MEF2 gene family belongs to the family of MADS (MCM1, agamous, deficiens, serum response factor)-box transcription factors, MEF2 exist in a wide range of cardiac and skeletal muscle cells, is the first discovered DNA binding activity factor with the muscle property, is an important regulatory factor for muscle histogeny and development. It can control vertebrate muscle-specific gene expression, and it is important for the process of muscle fiber and nervous system development. Because of the MEF2 plays an essential roles in regulating cell multiplication and tissue differentiation, to further reveal the function and mechanism of MEF2, contribute to better understanding the occurrence and development mode of muscle tissue. In this study, cloning the MEF2A and analysis its structure by comparative genomics and bioinformatics methods, phylogenesis of MEF2A also be analyzed by drawing Phylogentic tree; Expression of MEF2A gene and MEF2 gene family in different tissues and diferent period on large white pigs were analyzed by Real time PCR; The main results are as follows:1. MEF2A gene of pig was cloned (Genbank Accession Number: EF576923). The coding region of pig MEF2A gene is composed of 1524bp encoding 508 AA, and molecular weight is 50844.98 Da. There is a MADS-box domain in the vicinity of 10-243.2. There find a MADS-box domain by comparison of homology in the pig MEF2 gene family. The comparison of homology on MADS-box domain in four genes show that: the homology of MEF2B and MEF2D is 100%; MEF2A has large differentiation level, the homology with MEF2B and MEF2D is 89%.3. Based on the cDNA sequence of pig MEF2A gene, phylogenesis of nine species MEF2A be analyzed by drawing Phylogentic tree with Neighbor-Joining method.4. Expression of MEF2A gene in 10 tissues (Heart, Liver, Stomach, Spleen, Kidney, Lung, Large Intestine, Small Intestine, Dorsal muscle, Lower Limb muscle ) on 90, 180, 270-day-old large white pigs were analyzed by Real time PCR. The results show that the expression of MEF2A gene in muscle tissue is high and stable, and other organizations is relatively low, Liver is lowest.5. Expression of MEF2 gene family in 10 tissues on 90-day-old large white pigs were analyzed by the same mothed. The results showed that expression of four genes with common features: There is high expression in muscle tissue, eg: Dorsal muscle, Lower Limb muscle and Heart muscle; the second is visceral muscle, eg: Stomach, Large Intestine and Small Intestine; low expression in Liver, Kidney and Lung。At same time,compared the expression of MEF2 gene family in same tissue.