Equine Influenza Virus Subtype H7N7 Monoclonal Antibody Preparation And Identification Of Xinjiang Strain

Equine influenza shorter for EIV,is that the reason is gluing virus section the (Orthomyxoviridae) influenza virus belongs to the(Influenzavirus) horse A type influenza virus (EIV) arouses one kind of dyadic popular impatient outbreak infectious disease of Equus animal. Horse flu is altitude get in touch with,pneogaster infectious disease and horse nose pneumonia virus,horse arteritis virus become that the horse is the most important three kind pneogaster pathogene.One kind,propagation are prompt,assume urgency being popular for with other influenza virus,provisions Ma Ye and horse race job have brought about certain loss equally. Therefore,diagnose,ascertain aetiology if being able to carry out a fleetness at the right moment, in if the body initial stage being in popular being to be able to check going into action have infected EIV,be a fleetness thereby deal with case or early phase takes precautions against in advance treat provide the diagnose basis,the high limit field cuts down economic losses,appear especially important right away.For this purpose,originally,study the monoclonal antibody drafting by developing out sub EIV H7N7 type peculiarity,be that preparation horse flu diagnose reagent has establish a basis then.Sub method purification H7N7 type horse influenza virus studying density grad is at odds with the community with cane sugar originally,respectively,the immune Balb/C small rat queen fetches the spleen cell and SP2/0 fuses,preparation one horse influenza virus peculiarity monoclonal antibody 1C6.Liquid has no cross reaction without exception by the fact that ELISA and Western-blot method identifying certificate IC6 Shan resisting and the horse infecting nature anaemia virus(EIAV),horse arteritis virus(EAV),regular chicken embryo urine bag,1C6 ascites ELISA effect price is 1:1.6 X 104.Sub type of H7N7 mAb studying success develops out resisting originally,analysis,serum mimic the antigenicity being EIV diagnosing,vaccine mass monitors to reach an epidemiology waiting to have provided condition on inquiry.On October,2007,the plain plans preparation H3N8 type horse flu peculiarity monoclonal antibody,large-scale horse flu,separation and first step having changed focal point of the work to the horse influenza virus right away identify a job since Xinjiang,Inner Mongolia,fields such as Gansu Province,Wuhan have broken out.Xinjiang area is the first distance of outburst of equine influenza,will airlift the cotton swab sample returning inoculating with the age SPF chicken on 9th embryo by that a series handles the queen,area parts for together in Xinjiang to 8 horse influenza viruses,the first step having been in progress with RT—PCR method identifies,identifies for H3N8 type horse and an influenza virus.Monoclonal antibody has provided new aetiology to preparation H3N8 type equine influenza,has had a pertinency to the building-up diagnosing method more.