Three Fritillaria.spp Tissure Culture And Study On Conservation Technology

In this experiment,effective multiplication method,rooting and bulblet formation and germplasm conservation in vitro were studied based on onamental Fritillaria of three varieties-F.imperialis,F.acmopetala,F.persica.In order to establish the propagation system for appliance and extend of ornamental fritillarias.The main results are as followings:1.Effective multiplication method was set up.In the period found of axenic clone 10%NaClO and 0.1% HgCl2 is the best disinfector to deal with the bulb,as to neat part, such as leave, filament, and bulbil is fit to use 10%Naclo 18～20mins.And found the best effect is the bulb scale with base and filament,leaf was not inducement adventitious buds.Free leaf of F. imperialis is induced better.F.imperialis is fit MS+BA1.0mg/L+NAA0.2mg/L,F.acmopetala is fit MS+ BA1.0mg/L+NAA0.1mg/L,free leaf of F.imperialis is fit MS+BA2.0mg/L+NAA0.5mg/L,but the medi-um fit to culture F.persica haven't been found.2. In the period of Subculture-Found of mass production,the most multiplication of adventitious buds in MS+BA0.5mg/L+NAA 0.1mg/L during subculture of F.imperialis and F.acmopetala.3. Stage of rooting and bulblet formation.It was favorable for rooting and bulblet formation of F.acmopetala in reducing the concentration of macroelement.1/4MS proved the best concentration.NAA promoted rooting but made against forming bulblet.Sucrose can help to form bulblet, the best concentration was 60g/L.4. Transplanting.Transplanting substrate with Peat:Longkang Gray:perlite=7:2:1 was the best,where the survival rate could reach 100%.5.The conservation of Fritillaria plantlets.The results showed that,MS or half strength MS medium with 1.0～3.0mg/L ABA;the MS medium with 70～90g/L sucrose restricted in vitro growth of the shoots efficiently and prolonged the interval between subcultures over 9 months.Otherwise,10～50g/L mannitol could not restrict the growth of in vitro shoots effectively.