| Lily becomes one of the popular bulbous flowers in the cut flower market. China is one of the origin country of lily resources, but, lily breeding work begins later in our country. In order to speed up the breeding work in china, it is valuable to utilize the excellent species which introduced into our country breeding new cultivars. In this paper, tens of introduced excellent cultivars were collected as the materials, the lily crossbreeding, hybrid embryo rescue culture and hybrid identification in the early time were studied. The results were as follow:The pollen germination rates of'Calvados','Action','Siberia','Aktiva','Prato','Brunello'all above 60%, the highest is'Action', reach up to 90.1%, they all are good male materials.'Pollyanna'has the lowest pollen germination rate, which is 40.93%. Fruit set percentage and percentage of embryo are good indices to estimate the cross-compatibility. The twelve cross combination shows that oriental lily hybrids have a good cross-compatibility, fruit set percentage of'Calvados'above 50%,when as the female material, percentage of embryo reach up to 10% also,'Siberia'cross-compatibility is poorest as female materials. Asiatic lily hybrids cross-compatibility have more variation ,'Pollyanna'×'Brunello'has the highest fruit set percentage, reach up to 26.17%, the percentage of embryo is 39.29%,'Prato'×'Pollyanna'has none embryo produced. Distant cross-combination'White Heaven'×'Siberia'and'White Heaven'×'Aktiva'both have no hybrids embryo produce.Pollination at the stigma were useful to'Calvados'×'Siberia','Pollyanna'×'Brunello',the fruit set percentage and percentage of embryo reach up to 99.33% and 85.3% of the former, and the latter one reach up to 74.00% and 21.26%. 12 methods varies at pollination time and stigma mucus were not overcoming cross-incompatibility at'Elegance'×'Sorbonne'.Capsule culture is more useful than ovary-slice culture until 45 days after pollination. The capsule culture of'Aktiva'×'Siberia'obtained 22 embryos,'Medusa'×'Janna'obtained 24 embryos. The time of in vitro culture of hybrids capsule can be reach to 14 days after pollination earliest, however, 21 days was good for capsule growth in the future, the survival rates reach up to 100%, when MS culture medium added 9% sucrose concentration and 1.0mg.L-1 NAA.Seven medium used to do embryo culture were tested, MS+0.01mg.L-1NAA+3% or 6% sucrose was good for bulblets formation, while MS+0.1 mg.L-1NAA+0.5 /1.0 mg.L-1BA+3% or 6% sucrose was beneficial to propagation efficiency in a long time. Darkness culture for a time about 7～21 days after stripping could increase the seedling height and the amount of roots of hybrid plantlets.SRAP molecular marker technique were applied to F1 lily hybrids identification, 25ng.ul-1DNA were extracted from lily flesh leaf, 20ng DNA template was good for SRAP amplification, distinct bands of parental materials amplified in 16 pairs of SRAP primers from 56 pairs primer, they are:em1/me5; em1/me7; em1/me8; em2/me6; em3/me5; em3/me7; em4/me8; em5/me6; em5/me5; em5/me7; em5/me8; em6/me2; em6/me4; em6/me8; em7/me1; em7/me8。SRAP primer pair 40(em5/me8) amplified distinct bands of parental materials and F1 hybrids of'Calvados'×'Siberia', proved the 21 hybrids from embryo culture were true hybrids, cluster analysis of SRAP amplified bands proved the hybrids lies between parents genetic distance. SRAP primer pair 14(em2/em6)identified parental materials and F1 hybrids of'Siberia'×'Action'and'Action'×'Siberia', SRAP primer pair 44(em6/em4)identified'Calvados'×'Action'and'Action'×'Calvados'. |
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