| Suis(SS)is an important pathogen of human being and animal.Streptococcus is an important zoonosis bacteria that can cause pigs Septicemia,meningitis,Pneumonia, multi-arthritis and multi-polySerositis.Once human being suffered from the type 2 Streptococcus,it will Show serious clinical symptom and death with its toxin factor.The paper dives it particular summarizing from epidemiology,pathogeny and its virulence factors,nosogenesis,medicine sensitivity and its prevention.And the paper also expounds that nosogenesis and prevention of Streptococcus in Shijiazhuang by the three tests ot those Isolation and Pathogeny Identificatlon,8 virulence factors were selected to be detected by PCR assay and tile establishment of Multiplex PCR of serotype 1,2,7,9 of S.suis.First of all,from those suspicious:samples-in hose from the different district of Shijiazhuang,they were found 72 positive samples which by the direct microscopical check and sample culture microscopical check.Though its isolation,those of 18 typical separating trunks were identified and shew that the cause of the disease of streptococcus in Shijiazhuang focused on C group(9/18),D group(6/18),G group(1/18)and two unidentified strains(2/18),and with its no obvious distribution figure.The distribution of virulence-associate factors of Shijiazhuang isolates was unknown. To investigate the distribution of virulence associate factors of Streptococcus suis(SS),8 virulence factors were selected to be detected by PCR assay.The results showed that 21 virulent isolates of SS2 in the different district of China were detected in this assay.The positive detection rates of cps2/gdh+/gapdh+/ef+/mrp+/sly+/fbps+/orf2+ were 90.5% (19/21);The results showed the distribution of virulent associate factors of virulent streptococcus suis in Shijiazhuang was different from isolates of the other district of China.The virulence associate factors profile was conform,which suggested that only the 10%strains of SS2 in Shijiazhuang was virulent stains which have all 8 virulence factors,the most part of strains of SS2 in Shijiazhuang was low virulent stains which do not have all 8 virulence factors.Five pairs of primers were designed,in which 4 pairs of them were based on capsular polysaccharide(cps)biosynthesis genes specific for serotype 1,2,7 and 9,the other pairs of them were based on the glutamate dehydrogenase(gdh)gene specific for S.suis,and they were detected by multiplex PCR assays.After serial tests,multiplex PCR of serotype 1,2,7,9 were established successfully.In the serial tests,the specific assays of multiplex PCR was demonstrated by detecting the 35 serotypes of S.suis and one strain of Streptococcus group B,one strain of Streptococcus group C and one strain of Streptococcus group D,and other four strains,and by sequencing the products,of multiplex PCR;in the sensitive assays,multiplex PCR of S.suis serotype 1,2,71and 9 could be detected definitely when the template contained as few as 100 cfu;multiplex PCR of serotype 2 was used to detect 18 strains of serotype 2 known,its coincidence rate is 100%.360 palatine tonsil samples of clinical healthy pigs and 136 tissue samples of diseased pigs were collected from the different district of Shijiazhuang.By means of multiplex PCR of S.suis serotype 1,2,7 and 9 assays and plate agglutination test,54 strains of S.suis,in which 8 strains belonged to serotype 2,3 strains belonged to serotype 7, 3strains belonged to serotype 9,none of the strains were SS1,and forty unidentified strains,were detected and identified from the 360 palatine tonsils.The detection rate of S.suis hit 15.0%.24 strains of S.suis were detected and identified from the 136 tissue samples and 2 strains were serotype 2,1 strains were serotype 7,1 strains were serotype 9,the others are not serotype 1,2,1/2,7,9,14. |
